|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG1, kappa|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.125 µg/ml|
|Functional Assay (FN)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.
Applications Reported: This 11B11 antibody has been reported for use in cytokine neutralization, ELISPOT, and ELISA cap.
Applications Tested: The Functional Grade Purified 11B11 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-4 bioactivity.
The 11B11 antibody at 0.125 µg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-4 in a CTLL-2 cell proliferation assay. Please refer to Best Protocols for more information.
The 11B11 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-4 (IL-4) in combination with the biotinylated BVD6-24G2 (cat. 13-7042) antibody for detection and recombinant mouse IL-4 (cat. 14-8041) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.
Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.
Storage and handling: Use in a sterile environment.
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.