Invitrogen

IL-4 Monoclonal Antibody (BVD4-1D11)

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IL-4 Antibody in Flow Cytometry (Flow) — 4 Effect of TNF-alpha or LPS on preference of BC1 for Th differentiation. DO11.10 CD4 + T cells were co-cultured with unstimulated BC1 cells (iDC), BC1 cells matured by TNF-alpha or LPS (TNF/DC or LPS/DC) in the presence of a high- or low-dose OVA 323-339 peptide. The cells were stained by KJ1-26 mAb and monitored for intracellular staining of IFN-gamma and IL-4. (a) Dot plots gated by KJ1-26 positive cells. Results are representative of 10 independent experiments. (b) Proportions of IFN-gamma + IL-4 - or IL-4 + IFN-gamma - cells in the KJ1-26 positive cells. Mean +- SE of 10 independent experiments. Statistical significance was calculated by the Student's t -test (** P < 0*01).

Product Details

AMC0042

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	Assay-dependent	View 3 publications 3 publications
ELISA (ELISA)	Assay-dependent	View 7 publications 7 publications
Neutralization (Neu)	Assay-dependent	-
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2b
Class	Monoclonal
Type	Antibody
Clone	BVD4-1D11
Immunogen	Recombinant mouse IL-4.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	purified
Storage buffer	PBS
Contains	no preservative
Storage conditions	-20°C
RRID	AB_2536384

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IL-7Rα Expression Regulates Murine Dendritic Cell Sensitivity to Thymic Stromal Lymphopoietin.

Journal of immunology (Baltimore, Md. : 1950)

Kummola L,Ortutay Z,Chen X,Caucheteux S,Hämäläinen S,Aittomäki S,Yagi R,Zhu J,Pesu M,Paul WE,Junttila IS

AMC0042 was used in Flow cytometry/Cell sorting to show that murine ex vivo splenic DCs are unresponsive to TSLP, as they fail to phosphorylate STAT5, but in vitro overnight culture, especially in presence of IL-4, renders DCs responsive to both TSLP and IL-7.

Mon May 15 00:00:00 EDT 2017

Alternaria-induced release of IL-18 from damaged airway epithelial cells: an NF-κB dependent mechanism of Th2 differentiation?

PloS one

Murai H,Qi H,Choudhury B,Wild J,Dharajiya N,Vaidya S,Kalita A,Bacsi A,Corry D,Kurosky A,Brasier A,Boldogh I,Sur S

AMC0042 was used in ELISA to study the mechanism of alternaria-mediated damage in airway epithelial cells

Tue Jul 31 00:00:00 EDT 2012

Humoral and cytokine response during protection of mice against secondary hydatidosis caused by Echinococcus granulosus.

Parasitology research

Al-Qaoud KM,Abdel-Hafez SK

AMC0042 was used in ELISA to induce protective immunity against secondary hydatidosis and elucidate the mechanisms of protection

Thu Dec 01 00:00:00 EST 2005

Negative feedback regulation of T helper type 1 (Th1)/Th2 cytokine balance via dendritic cell and natural killer T cell interactions.

Blood

Minami K,Yanagawa Y,Iwabuchi K,Shinohara N,Harabayashi T,Nonomura K,Onoé K

AMC0042 was used in flow cytometry to examine the effects of the T helper type 1 and 2-cytokine environment on dendritic cell induction of natural killer T cell-mediated cytokine production

Thu Sep 01 00:00:00 EDT 2005

Social isolation stress exacerbates autoimmune disease in MRL/lpr mice.

Journal of neuroimmunology

Chida Y,Sudo N,Kubo C

AMC0042 was used in ELISA to investigate how isolation stress exacerbates autoimmune disease in MRL/lpr mice

Sat Jan 01 00:00:00 EST 2005

Arthropod-derived histamine-binding protein prevents murine allergic asthma.

Journal of immunology (Baltimore, Md. : 1950)

Couillin I,Maillet I,Vargaftig BB,Jacobs M,Paesen GC,Nuttall PA,Lefort J,Moser R,Weston-Davies W,Ryffel B

AMC0042 was used in ELISA to test if complete neutralization of histamine by an arthropod-derived, high affinity histamine-binding protein prevents allergic asthma

Wed Sep 01 00:00:00 EDT 2004

A transgenic mouse model for T-cell ignorance of a glial autoantigen.

Journal of autoimmunity

Cabarrocas J,Piaggio E,Zappulla JP,Desbois S,Mars LT,Lassmann H,Liblau RS

AMC0042 was used in ELISA to determine the fate of autoreactive CD4+T cells in HNT-TCR x GFAP-HA double transgenic mice

Sat May 01 00:00:00 EDT 2004

Assay system for the effect of prostaglandin E(2) in the determination of polarized cytokine production.

Methods in molecular biology (Clifton, N.J.)

Kuroda E,Yamashita U

AMC0042 was used in ELISA to examine the effect of prostaglandin E(2) on polarized cytokine production

Mon Feb 09 00:00:00 EST 2004

Mouse V alpha 14i natural killer T cells are resistant to cytokine polarization in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Matsuda JL,Gapin L,Baron JL,Sidobre S,Stetson DB,Mohrs M,Locksley RM,Kronenberg M

AMC0042 was used in flow cytometry to study V alpha 14i NKT cells.

Tue Jul 08 00:00:00 EDT 2003

Tumour necrosis factor-alpha but not lipopolysaccharide enhances preference of murine dendritic cells for Th2 differentiation.

Immunology

Kikuchi K,Yanagawa Y,Aranami T,Iwabuchi C,Iwabuchi K,Onoé K

AMC0042 was used in flow cytometry to determine the influences of maturational condition and antigen dose on the capability of DC to induce helper T-cell (Th) differentiation.

Wed Jan 01 00:00:00 EST 2003

Multiple Chlamydia pneumoniae antigens prime CD8+ Tc1 responses that inhibit intracellular growth of this vacuolar pathogen.

Journal of immunology (Baltimore, Md. : 1950)

Wizel B,Starcher BC,Samten B,Chroneos Z,Barnes PF,Dzuris J,Higashimoto Y,Appella E,Sette A

AMC0042 was used in ELISA to elucidate the role of CD8(+) T cells in immunity to Chlamydia pneumoniae.

Sun Sep 01 00:00:00 EDT 2002

Single cell analysis reveals that IL-4 receptor/Stat6 signaling is not required for the in vivo or in vitro development of CD4+ lymphocytes with a Th2 cytokine profile.

Journal of immunology (Baltimore, Md. : 1950)

Jankovic D,Kullberg MC,Noben-Trauth N,Caspar P,Paul WE,Sher A

AMC0042 was used in flow cytometry to report that IL-4R/Stat6 signaling controls the final frequency of Th2 lymphocytes, but is not essential for Th2 cell development.

Wed Mar 15 00:00:00 EST 2000

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

Gene Aliases: BSF-1; Il-4; Il4

UniProt ID: (Mouse) P07750

Entrez Gene ID: (Mouse) 16189

Molecular Function: interleukin superfamily

Function(s)

Process(es)

cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity

IL-4 Monoclonal Antibody (BVD4-1D11)

Product Details

AMC0042

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

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