Invitrogen

IL-4 Monoclonal Antibody (BVD6-24G2), FITC, eBioscience™

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IL-4 Antibody in Flow Cytometry (Flow) — Mouse splenocytes were stimulated with ConA for 2 days, followed by Mouse IL-2 Recombinant Protein (Product # 14-8021-64) and Mouse IL-4 Recombinant Protein (Product # 14-8041-80) for 3 days, and re-stimulated with immobilized Anti-Mouse CD3 Functional Grade Purified (Product # 16-0032-82) and soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) in the presence of Brefeldin A (Product # 00-4506-51) for 5 hours. Cells were surface stained with Anti-Mouse CD4 PE (Product # 12-0041-82) and intracellularly stained with Anti-Mouse IL-4 FITC.

IL-4 Antibody in ELISA (ELISA) — Figure 4 Airway allergy is induced by Der f 31. Four- to 7-week-old female BALB/c mice were immunized with PBS, Der f 31 or Der f 1 and then challenged with Der f 31 or Der f 1 for one week. ( A ) The scheme of establishment of mouse model. ( B ) Total IgE, allergen-specific IgE, IgG1 and IgG2a were detected by ELISA by optical density (OD). ( C ) The cytokines in BALF. ( D ) The cytokines in the lung homogenates. E, Histological sections of lung tissues. These data (means +- SEMs) were generated from one of three experiments (n = 6) and processed with GraphPad software. The significant differences between two groups were tested by two-tailed T-test. **p < 0.01. ***p < 0.001. ns, no significant difference.

Product Details

11-7042-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.5 µg/test	View 18 publications 18 publications
ELISA (ELISA)	-	View 1 publication 1 publication
Functional Assay (FN)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	BVD6-24G2
Immunogen	E.coli expressed mouse IL-4 (gluteraldehyde XL)
Conjugate	FITC Biotin PE-Cyanine7 Unconjugated
Excitation/Emission Max	498/517 nm View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_465388

Product Specific Information

Description: The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.

Applications Reported: BVD6-24G2 has been reported for use in intracellular flow cytometric analysis.

Applications Tested: This BVD6-24G2 antibody is offered in 2 formats: - µg size: has been tested by intracellular staining and flow cytometric analysis of restimulated mouse splenocytes cultures. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of restimulated mouse splenocytes cultures. Refer to catalog number suffix on the vial for amount to use per test: -41 is 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

NLRX1 is a key regulator of immune signaling during invasive pulmonary aspergillosis.

PLoS pathogens

Kastelberg B,Tubau-Juni N,Ayubi T,Leung A,Leber A,Hontecillas R,Bassaganya-Riera J,Kale SD

Published figure using IL-4 monoclonal antibody (Product # 11-7042-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Differential Activation of Unconventional T Cells, Including iNKT Cells, in Alcohol-Related Liver Disease.

Alcoholism, clinical and experimental research

Marrero I,Maricic I,Morgan TR,Stolz AA,Schnabl B,Liu ZX,Tsukamoto H,Kumar V

11-7042-82 was used in Flow Cytometry to investigate the role of innate-like unconventional T cells in alcohol-related liver disease.

Fri May 01 00:00:00 EDT 2020

The NDV-3A vaccine protects mice from multidrug resistant Candida auris infection.

PLoS pathogens

Singh S,Uppuluri P,Mamouei Z,Alqarihi A,Elhassan H,French S,Lockhart SR,Chiller T,Edwards JE,Ibrahim AS

11-7042-82 was used in Flow Cytometry to perform bioinformatic studies to identify the presence of adhesin proteins in C. auris, with sequence as well as 3-D structural homologies to the major adhesin/invasin of C. albicans, Als3.

Thu Aug 01 00:00:00 EDT 2019

Oral administration of taheebo (Tabebuia avellanedae Lorentz ex Griseb.) water extract prevents DSS-induced colitis in mice by up-regulating type II T helper immune responses.

BMC complementary and alternative medicine

Park HJ,Lee SW,Kwon DJ,Heo SI,Park SH,Kim SY,Hong S

11-7042-82 was used in Flow Cytometry to show that taheebo water extract (TWE) is an excellent natural product with therapeutic effects to help improve inflammatory disorders such as colitis.

Wed Sep 06 00:00:00 EDT 2017

Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells.

Scientific reports

Wang H,Lin J,Zeng L,Ouyang C,Ran P,Yang P,Liu Z

Thu Aug 17 00:00:00 EDT 2017

Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells.

Scientific reports

Wang H,Lin J,Zeng L,Ouyang C,Ran P,Yang P,Liu Z

Thu Aug 17 00:00:00 EDT 2017

Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.

Acta pharmacologica Sinica

Zhou YQ,Weng XF,Dou R,Tan XS,Zhang TT,Fang JB,Wu XW

Published figure using IL-4 monoclonal antibody (Product # 11-7042-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

T cell-intrinsic ASC critically promotes T(H)17-mediated experimental autoimmune encephalomyelitis.

Nature immunology

Martin BN,Wang C,Zhang CJ,Kang Z,Gulen MF,Zepp JA,Zhao J,Bian G,Do JS,Min B,Pavicic PG,El-Sanadi C,Fox PL,Akitsu A,Iwakura Y,Sarkar A,Wewers MD,Kaiser WJ,Mocarski ES,Rothenberg ME,Hise AG,Dubyak GR,Ransohoff RM,Li X

11704282 was used in flow cytometry to investigate the role of IL-1beta produced by a T helper 17 cell-intrinsic ASC-NLRP3-caspase-8 inflammasome in experimental autoimmune encephalomyelitis

Sun May 01 00:00:00 EDT 2016

Itk is required for Th9 differentiation via TCR-mediated induction of IL-2 and IRF4.

Nature communications

Gomez-Rodriguez J,Meylan F,Handon R,Hayes ET,Anderson SM,Kirby MR,Siegel RM,Schwartzberg PL

11-7042 was used in Flow cytometry/Cell sorting to investigate the role of IL-9 in allergic asthma and autoimmunity, showing that Itk is required for Th9 differentiation via TCR-mediated induction of IL-2 and IRF4.

Thu Mar 03 00:00:00 EST 2016

Obesity enhances Th2 inflammatory response via natural killer T cells in a murine model of allergic asthma.

International journal of clinical and experimental medicine

Chen YP,Zhang JH,Li CQ,Sun QX,Jiang XH

11-7042 was used in Flow cytometry/Cell sorting to investigate the mechanisms connecting the development of asthma and obesity.

Wed Dec 02 00:00:00 EST 2015

Anti-G-CSF treatment induces protective tumor immunity in mouse colon cancer by promoting protective NK cell, macrophage and T cell responses.

Oncotarget

Morris KT,Castillo EF,Ray AL,Weston LL,Nofchissey RA,Hanson JA,Samedi VG,Pinchuk IV,Hudson LG,Beswick EJ

11-7042 was used in Flow cytometry/Cell sorting to indicate a crucial role for G-CSF inhibition in promoting protective anti-tumor immunity.

Tue Sep 08 00:00:00 EDT 2015

Prophylaxis with a respiratory syncytial virus (RSV) anti-G protein monoclonal antibody shifts the adaptive immune response to RSV rA2-line19F infection from Th2 to Th1 in BALB/c mice.

Journal of virology

Boyoglu-Barnum S,Chirkova T,Todd SO,Barnum TR,Gaston KA,Jorquera P,Haynes LM,Tripp RA,Moore ML,Anderson LJ

11-7042 was used in Flow cytometry/Cell sorting to suggest that the RSV G protein not only contributes to disease, but also dampens the host immune response to infection.

Mon Sep 01 00:00:00 EDT 2014

Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.

Nature immunology

Islam SA,Chang DS,Colvin RA,Byrne MH,McCully ML,Moser B,Lira SA,Charo IF,Luster AD

11-7042 was used in Flow cytometry/Cell sorting to investigate the biological activity of CCL8, showing that it promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.

Tue Feb 01 00:00:00 EST 2011

PIPKI gamma 90 negatively regulates LFA-1-mediated adhesion and activation in antigen-induced CD4+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Wernimont SA,Legate KR,Simonson WT,Fassler R,Huttenlocher A

11-7042 was used in Flow cytometry/Cell sorting to demonstrate the activity of PIPKI gamma 90 as a novel negative regulator of antigen-induced CD4+ T cell adhesion and activation.

Fri Oct 15 00:00:00 EDT 2010

Protein kinase B/Akt signals impair Th17 differentiation and support natural regulatory T cell function and induced regulatory T cell formation.

Journal of immunology (Baltimore, Md. : 1950)

Pierau M,Engelmann S,Reinhold D,Lapp T,Schraven B,Bommhardt UH

11-7042 was used in Flow cytometry/Cell sorting to indicate an important role of PKB signals on control of TGF-beta1-mediated T cell responses and, thereby, on tolerizing and inflammatory immune processes.

Sun Nov 15 00:00:00 EST 2009

Vaccine-induced antibody isotypes are skewed by impaired CD4 T cell and invariant NKT cell effector responses in MyD88-deficient mice.

Journal of immunology (Baltimore, Md. : 1950)

Iweala OI,Smith DW,Matharu KS,Sada-Ovalle I,Nguyen DD,Dekruyff RH,Umetsu DT,Behar SM,Nagler CR

11-7042 was used in Flow cytometry/Cell sorting to determine how the adaptive immune response generated to this live attenuated vaccine is regulated, which may have important implications for vaccine design.

Sat Aug 15 00:00:00 EDT 2009

Infection with a helminth parasite attenuates autoimmunity through TGF-beta-mediated suppression of Th17 and Th1 responses.

Journal of immunology (Baltimore, Md. : 1950)

Walsh KP,Brady MT,Finlay CM,Boon L,Mills KH

11-7042 was used in Flow cytometry/Cell sorting to provide evidence that parasites can exert bystander immunosuppression of pathogenic T cells that mediate autoimmune diseases.

Sat Aug 01 00:00:00 EDT 2009

Galectin-1 functions as a Th2 cytokine that selectively induces Th1 apoptosis and promotes Th2 function.

European journal of immunology

Motran CC,Molinder KM,Liu SD,Poirier F,Miceli MC

11-7042 was used in Flow cytometry/Cell sorting to identify galectin-1 as a cross-regulatory cytokine that selectively antagonizes Th1 survival, while promoting TCR-induced Th2 cytokine production.

Sat Nov 01 00:00:00 EDT 2008

Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance.

International immunology

Ito H,Ando K,Ishikawa T,Nakayama T,Taniguchi M,Saito K,Imawari M,Moriwaki H,Yokochi T,Kakumu S,Seishima M

11-7042 was used in Flow cytometry/Cell sorting to open up a new method for clearing the virus from patients with persistent HBV infection.

Tue Jul 01 00:00:00 EDT 2008

Phenotypic differences between Th1 and Th17 cells and negative regulation of Th1 cell differentiation by IL-17.

Journal of leukocyte biology

Nakae S,Iwakura Y,Suto H,Galli SJ

11-7042 was used in Functional assays to compare the cell surface marker expression profiles of Th1 and Th17 cells.

Tue May 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

Gene Aliases: BSF-1; Il-4; Il4

UniProt ID: (Mouse) P07750

Entrez Gene ID: (Mouse) 16189

Molecular Function: interleukin superfamily

Function(s)

Process(es)

cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity

IL-4 Monoclonal Antibody (BVD6-24G2), FITC, eBioscience™

Product Details

11-7042-82

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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