Invitrogen

IL-4 Monoclonal Antibody (BVD6-24G2), Biotin, eBioscience™

Product Image Gallery

IL-4 Antibody in Flow Cytometry (Flow) — Staining of mouse splenocytes stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized Anti-Mouse CD3 Functional Grade Purified (Product # 16-0031-82) and soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) in the presence of Brefeldin A for 5 hours with Anti-Mouse CD4 PE (Product # 12-0041-82) and intracellularly stained with Anti-Mouse IL-4 FITC.

IL-4 Antibody in ELISA (ELISA) — Figure 4 Airway allergy is induced by Der f 31. Four- to 7-week-old female BALB/c mice were immunized with PBS, Der f 31 or Der f 1 and then challenged with Der f 31 or Der f 1 for one week. ( A ) The scheme of establishment of mouse model. ( B ) Total IgE, allergen-specific IgE, IgG1 and IgG2a were detected by ELISA by optical density (OD). ( C ) The cytokines in BALF. ( D ) The cytokines in the lung homogenates. E, Histological sections of lung tissues. These data (means +- SEMs) were generated from one of three experiments (n = 6) and processed with GraphPad software. The significant differences between two groups were tested by two-tailed T-test. **p < 0.01. ***p < 0.001. ns, no significant difference.

Product Details

13-7042-85

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	Assay-dependent	View 3 publications 3 publications
ELISA (ELISA)	0.5-2 µg/mL	View 14 publications 14 publications
Functional Assay (FN)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	BVD6-24G2
Conjugate	Biotin FITC PE-Cyanine7 Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_466903

Product Specific Information

Description: The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.

Applications Reported: The BVD6-24G2 antibody is used for detection of mouse IL-4 by ELISA.

Applications Tested: The BVD6-24G2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse Interleukin-4 (IL-4) in combination with the purified 11B11 (14-7041) antibody for capture and recombinant mouse IL-4 (14-8041) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tumor suppressor p53 regulates intestinal type 2 immunity.

Nature communications

Chang CY,Wang J,Zhao Y,Liu J,Yang X,Yue X,Wang H,Zhou F,Inclan-Rico JM,Ponessa JJ,Xie P,Zhang L,Siracusa MC,Feng Z,Hu W

13-7042-85 was used in ELISA to demonstrate that p53 plays a pivotal role in intestinal type 2 immunity during parasitic infection.

Mon Jun 07 00:00:00 EDT 2021

DEK-targeting aptamer DTA-64 attenuates bronchial EMT-mediated airway remodelling by suppressing TGF-β1/Smad, MAPK and PI3K signalling pathway in asthma.

Journal of cellular and molecular medicine

Song Y,Wang Z,Jiang J,Piao Y,Li L,Xu C,Piao H,Li L,Yan G

Published figure using IL-4 monoclonal antibody (Product # 13-7042-81) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

NLRX1 is a key regulator of immune signaling during invasive pulmonary aspergillosis.

PLoS pathogens

Kastelberg B,Tubau-Juni N,Ayubi T,Leung A,Leber A,Hontecillas R,Bassaganya-Riera J,Kale SD

Published figure using IL-4 monoclonal antibody (Product # 13-7042-81) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

The Journal of experimental medicine

Carpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C

14-5931 was used in ELISA to show the role of MKP5 in restraining p38 MAPK-mediated neutrophil oxidant production, thereby preventing LPS-induced vascular injury in mice.

Mon Apr 02 00:00:00 EDT 2018

Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells.

Scientific reports

Wang H,Lin J,Zeng L,Ouyang C,Ran P,Yang P,Liu Z

Published figure using IL-4 monoclonal antibody (Product # 13-7042-81) in ELISA

Thu Aug 17 00:00:00 EDT 2017

Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.

Acta pharmacologica Sinica

Zhou YQ,Weng XF,Dou R,Tan XS,Zhang TT,Fang JB,Wu XW

Published figure using IL-4 monoclonal antibody (Product # 13-7042-81) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Altered Glycosylation Patterns Increase Immunogenicity of a Subunit Hepatitis C Virus Vaccine, Inducing Neutralizing Antibodies Which Confer Protection in Mice.

Journal of virology

Li D,von Schaewen M,Wang X,Tao W,Zhang Y,Li L,Heller B,Hrebikova G,Deng Q,Ploss A,Zhong J,Huang Z

13-7042 was used in an ELISpot assay to identify a promising broadly protective HCV vaccine candidate that should be considered for further preclinical and clinical development.

Thu Dec 01 00:00:00 EST 2016

Mucosal Langerhans Cells Promote Differentiation of Th17 Cells in a Murine Model of Periodontitis but Are Not Required for Porphyromonas gingivalis-Driven Alveolar Bone Destruction.

Journal of immunology (Baltimore, Md. : 1950)

Bittner-Eddy PD,Fischer LA,Kaplan DH,Thieu K,Costalonga M

13-7042 was used in an ELISpot assay to indicate that both Langerhans cells and Th17 cells are redundant in contributing to alveolar bone destruction in a murine model of periodontitis.

Mon Aug 15 00:00:00 EDT 2016

A Natural Variant of the T Cell Receptor-Signaling Molecule Vav1 Reduces Both Effector T Cell Functions and Susceptibility to Neuroinflammation.

PLoS genetics

Kassem S,Gaud G,Bernard I,Benamar M,Dejean AS,Liblau R,Fournié GJ,Colacios C,Malissen B,Saoudi A

13-7042 was used in Flow cytometry/Cell sorting to highlight the importance of Vav1 adaptor functions in inflammatory cytokine production by effector T cells and susceptibility to neuroinflammation.

Fri Jul 01 00:00:00 EDT 2016

Non-glycosidic compounds can stimulate both human and mouse iNKT cells.

European journal of immunology

Jukes JP,Gileadi U,Ghadbane H,Yu TF,Shepherd D,Cox LR,Besra GS,Cerundolo V

13-7042 was used in an ELISA assay to investigate novel non-glycosidic ThrCer-based analogs that have improved potency in iNKT-cell activation compared with that of α-GalCer, and are clinically relevant iNKT-cell agonists.

Sun May 01 00:00:00 EDT 2016

Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis.

The Journal of allergy and clinical immunology

Bartnikas LM,Gurish MF,Burton OT,Leisten S,Janssen E,Oettgen HC,Beaupré J,Lewis CN,Austen KF,Schulte S,Hornick JL,Geha RS,Oyoshi MK

13-7042 was used in an ELISA assay to explore whether epicutaneous sensitization with food antigen predisposes to IgE-mediated anaphylaxis on oral allergen challenge.

Fri Feb 01 00:00:00 EST 2013

The transcription factor E4BP4 regulates the production of IL-10 and IL-13 in CD4+ T cells.

Nature immunology

Motomura Y,Kitamura H,Hijikata A,Matsunaga Y,Matsumoto K,Inoue H,Atarashi K,Hori S,Watarai H,Zhu J,Taniguchi M,Kubo M

13-7042 was used in an ELISA assay to indicate that E4BP4 has multiple functions in controlling the plasticity of IL-13 in Th1 cells, and IL-10 in Th1, Th2, Treg and NKT cells.

Sun May 01 00:00:00 EDT 2011

Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization.

Journal of immunology (Baltimore, Md. : 1950)

Weber KS,Hildner K,Murphy KM,Allen PM

13-7042 was used in an ELISpot assay to study the regulation of unique Th cell subset calcium signals and their correlation to biological functions, showing that Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization.

Wed Sep 01 00:00:00 EDT 2010

Human bone marrow-derived mesenchymal stem cells induce Th2-polarized immune response and promote endogenous repair in animal models of multiple sclerosis.

Glia

Bai L,Lennon DP,Eaton V,Maier K,Caplan AI,Miller SD,Miller RH

13-7042 was used in an ELISpot assay to suggest that bone marrow-derived mesenchymal stem cells represent a viable option for therapeutic approaches to multiple sclerosis.

Sat Aug 15 00:00:00 EDT 2009

MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.

Nature immunology

Perrigoue JG,Saenz SA,Siracusa MC,Allenspach EJ,Taylor BC,Giacomin PR,Nair MG,Du Y,Zaph C,van Rooijen N,Comeau MR,Pearce EJ,Laufer TM,Artis D

13-7042 was used in an ELISA assay to suggest that major histocompatibility complex class II-dependent interactions between basophils and CD4+ T cells promote T helper type 2 cytokine responses and immunity to helminth infection.

Wed Jul 01 00:00:00 EDT 2009

Peripheral dendritic cells are essential for both the innate and adaptive antiviral immune responses in the central nervous system.

Virology

Steel CD,Hahto SM,Ciavarra RP

13-7042 was used in an ELISpot assay to study the adaptive primary antiviral immune response in the CNS and the effect of dendritic cells upon this in vivo.

Sat Apr 25 00:00:00 EDT 2009

Estradiol-induced vaginal mucus inhibits antigen penetration and CD8(+) T cell priming in response to intravaginal immunization.

Vaccine

Seavey MM,Mosmann TR

13-7042 was used in an ELISpot assay to investigate the effect of estradiol during intravaginal immunisation, showing that estradiol-induced vaginal mucus inhibits antigen penetration and CD8(+) T cell priming.

Tue Apr 14 00:00:00 EDT 2009

Characterization of T-cell immunogenicity of two PE/PPE proteins of Mycobacterium tuberculosis.

Journal of medical microbiology

Chaitra MG,Shaila MS,Nayak R

13-7042 was used in Functional assays to demonstrate the immunogenicity of two PE/PPE proteins in BALB/c mice.

Mon Sep 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

Gene Aliases: BSF-1; Il-4; Il4

UniProt ID: (Mouse) P07750

Entrez Gene ID: (Mouse) 16189

Molecular Function: interleukin superfamily

Function(s)

Process(es)

cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity

IL-4 Monoclonal Antibody (BVD6-24G2), Biotin, eBioscience™

Product Details

13-7042-85

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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