Invitrogen

IL-4 Monoclonal Antibody (BVD6-24G2), eBioscience™

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IL-4 Antibody in Flow Cytometry (Flow) — 10.1371/journal.ppat.1008854.g006 Fig 6 Temporal immune responses by CD103+ DCs during in vitro fungal challenge assays. Freshly harvested AF293 conidia (5 X 10 5 ) were challenged against wild type and Nlrx1-/- CD103+ DCs (5 X 10 5 ) at 37degC at 5% CO 2 . Supernatant and cells were harvested immediately prior to challenge (0 hrs), and at 3, 6, 9, 12 hrs post challenge. (A) Percentage of wild type and Nlrx1-/- CD103+ DCs staining positive for IL-4 12 hours post treatment with killed hyphae and conidia. (B) Representative histograms and gating for IL-4 fluorescence. See S2 Fig for strategy. (C) Intracellular measurement of IL-4 by wild type and Nlrx1-/- CD103+ dendritic cells 12 hours post treatment with killed hyphae and conidia in the presence and absence of the JNK inhibitor (JNK-IN-8). (D) Western blot analysis of phosphorylated (-P) ERK1/2-P, P38-P, NFkappaB p65-P, JNK-P, cFos-P, JunB-P, Nlrx1, and beta-actin from wild type and Nlrx1-/- CD103+ dendritic cells during a 12-hour challenge against viable A . fumigatus conidia. Quantification of (E) JNK-P and (F) JunB-P. Measurement of secreted (G) RANTES (CCL5), (H) TARC (CCL17), (I) MDC (CCL22) by wild type and Nlrx1-/- CD103+ dendritic cells during a 24-hour challenge against viable A . fumigatus conidia. Asterisk denotes statistical significance, P < 0.05 Mann-Whitney U test. Error bars indicate standard deviation. N = 6-8 per experimental group.

IL-4 Antibody in ELISA (ELISA) — Figure 4 Airway allergy is induced by Der f 31. Four- to 7-week-old female BALB/c mice were immunized with PBS, Der f 31 or Der f 1 and then challenged with Der f 31 or Der f 1 for one week. ( A ) The scheme of establishment of mouse model. ( B ) Total IgE, allergen-specific IgE, IgG1 and IgG2a were detected by ELISA by optical density (OD). ( C ) The cytokines in BALF. ( D ) The cytokines in the lung homogenates. E, Histological sections of lung tissues. These data (means +- SEMs) were generated from one of three experiments (n = 6) and processed with GraphPad software. The significant differences between two groups were tested by two-tailed T-test. **p < 0.01. ***p < 0.001. ns, no significant difference.

Product Details

14-7042-85

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	Assay-Dependent	View 10 publications 10 publications
ELISA (ELISA)	-	View 2 publications 2 publications
Functional Assay (FN)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	BVD6-24G2
Conjugate	Unconjugated Biotin FITC PE-Cyanine7
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_468413

Product Specific Information

Description: The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.

Applications Reported: BVD6-24G2 is used for detection of mouse IL-4 by ELISA.

Applications Tested: The BVD6-24G2 antibody has been tested by sandwich ELISA for detection of mouse IL-4 in combination with 11B11 and can be used between 0.5-2 µg/mL.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NLRX1 is a key regulator of immune signaling during invasive pulmonary aspergillosis.

PLoS pathogens

Kastelberg B,Tubau-Juni N,Ayubi T,Leung A,Leber A,Hontecillas R,Bassaganya-Riera J,Kale SD

Published figure using IL-4 monoclonal antibody (Product # 14-7042-85) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells.

Scientific reports

Wang H,Lin J,Zeng L,Ouyang C,Ran P,Yang P,Liu Z

Published figure using IL-4 monoclonal antibody (Product # 14-7042-85) in ELISA

Thu Aug 17 00:00:00 EDT 2017

miR-451 limits CD4⁺ T cell proliferative responses to infection in mice.

Immunologic research

Chapman LM,Ture SK,Field DJ,Morrell CN

14-7042 was used in Flow cytometry/Cell sorting to demonstrate that miR-451 is a regulator of host immune responses to Plasmodium infection, particularly in the control of CD4+ T cell proliferation via a Myc-dependent pathway.

Tue Aug 01 00:00:00 EDT 2017

A non-canonical function of Ezh2 preserves immune homeostasis.

EMBO reports

Vasanthakumar A,Xu D,Lun AT,Kueh AJ,van Gisbergen KP,Iannarella N,Li X,Yu L,Wang D,Williams BR,Lee SC,Majewski IJ,Godfrey DI,Smyth GK,Alexander WS,Herold MJ,Kallies A,Nutt SL,Allan RS

14-7042 was used in Flow cytometry/Cell sorting to identify a chromatin-independent function of enhancer of zeste 2 that impacts on the development of the immune system.

Sat Apr 01 00:00:00 EDT 2017

Loss of Functionally Redundant p38 Isoforms in T Cells Enhances Regulatory T Cell Induction.

The Journal of biological chemistry

Hayakawa M,Hayakawa H,Petrova T,Ritprajak P,Sutavani RV,Jiménez-Andrade GY,Sano Y,Choo MK,Seavitt J,Venigalla RKC,Otsu K,Georgopoulos K,Arthur JSC,Park JM

14704285 was used in flow cytometry to assess the role of p38 signaling in limiting regulatory T cell induction

Fri Feb 03 00:00:00 EST 2017

Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.

Acta pharmacologica Sinica

Zhou YQ,Weng XF,Dou R,Tan XS,Zhang TT,Fang JB,Wu XW

Published figure using IL-4 monoclonal antibody (Product # 14-7042-85) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

ATP Binding Cassette Transporter ABCA7 Regulates NKT Cell Development and Function by Controlling CD1d Expression and Lipid Raft Content.

Scientific reports

Nowyhed HN,Chandra S,Kiosses W,Marcovecchio P,Andary F,Zhao M,Fitzgerald ML,Kronenberg M,Hedrick CC

14704285 was used in flow cytometry to show that ABCA7 regulates natural killer T cell development in a cell-extrinsic manner

Mon Jan 16 00:00:00 EST 2017

T cell-intrinsic ASC critically promotes T(H)17-mediated experimental autoimmune encephalomyelitis.

Nature immunology

Martin BN,Wang C,Zhang CJ,Kang Z,Gulen MF,Zepp JA,Zhao J,Bian G,Do JS,Min B,Pavicic PG,El-Sanadi C,Fox PL,Akitsu A,Iwakura Y,Sarkar A,Wewers MD,Kaiser WJ,Mocarski ES,Rothenberg ME,Hise AG,Dubyak GR,Ransohoff RM,Li X

14704285 was used in flow cytometry to investigate the role of IL-1beta produced by a T helper 17 cell-intrinsic ASC-NLRP3-caspase-8 inflammasome in experimental autoimmune encephalomyelitis

Sun May 01 00:00:00 EDT 2016

Cigarette smoke exposure exacerbates lung inflammation and compromises immunity to bacterial infection.

Journal of immunology (Baltimore, Md. : 1950)

Lugade AA,Bogner PN,Thatcher TH,Sime PJ,Phipps RP,Thanavala Y

14-7042 was used in an ELISpot assay to demonstrate multiple mechanisms by which cigarette smoke exposure predisposes patients to infection and contributes to mortality.

Sun Jun 01 00:00:00 EDT 2014

Antiviral drug ganciclovir is a potent inhibitor of microglial proliferation and neuroinflammation.

The Journal of experimental medicine

Ding Z,Mathur V,Ho PP,James ML,Lucin KM,Hoehne A,Alabsi H,Gambhir SS,Steinman L,Luo J,Wyss-Coray T

14-7042 was used in Flow cytometry/Cell sorting to suggest that the antiviral drug ganciclovir (GCV) may have beneficial effects in the CNS beyond its antiviral properties.

Mon Feb 10 00:00:00 EST 2014

The adaptor molecule signaling lymphocytic activation molecule (SLAM)-associated protein (SAP) is essential in mechanisms involving the Fyn tyrosine kinase for induction and progression of collagen-induced arthritis.

The Journal of biological chemistry

Zhong MC,Veillette A

14-7042 was used in Flow cytometry/Cell sorting to investigate how SAP plays an essential role in CIA because of Fyn-independent and Fyn-dependent effects on TFH cells and, possibly, other T cell types.

Fri Nov 01 00:00:00 EDT 2013

Resident renal mononuclear phagocytes comprise five discrete populations with distinct phenotypes and functions.

Journal of immunology (Baltimore, Md. : 1950)

Kawakami T,Lichtnekert J,Thompson LJ,Karna P,Bouabe H,Hohl TM,Heinecke JW,Ziegler SF,Nelson PJ,Duffield JS

14-7042 was used in Flow cytometry/Cell sorting to show that resident kidney MPCs comprise discrete subpopulations, producing anti-inflammatory and tissue-homeostatic factors to differing degrees.

Sun Sep 15 00:00:00 EDT 2013

Targeted delivery of lipid antigen to macrophages via the CD169/sialoadhesin endocytic pathway induces robust invariant natural killer T cell activation.

Proceedings of the National Academy of Sciences of the United States of America

Kawasaki N,Vela JL,Nycholat CM,Rademacher C,Khurana A,van Rooijen N,Crocker PR,Kronenberg M,Paulson JC

14-7042 was used in Functional assays to show that delivery of a lipid antigen to CD169(+) macrophages in vivo causes robust invariant natural killer T cell activation.

Tue May 07 00:00:00 EDT 2013

The proteasome inhibitor bortezomib inhibits T cell-dependent inflammatory responses.

Journal of leukocyte biology

Yanaba K,Yoshizaki A,Muroi E,Hara T,Ogawa F,Shimizu K,Sato S

14-7042 was used in Immunofluorescence to determine that bortezomib treatment induces T cell death, thereby suppressing CHS responses by reducing IFN-gamma production.

Thu Jul 01 00:00:00 EDT 2010

Small interfering RNA against transcription factor STAT6 inhibits allergic airway inflammation and hyperreactivity in mice.

Journal of immunology (Baltimore, Md. : 1950)

Darcan-Nicolaisen Y,Meinicke H,Fels G,Hegend O,Haberland A,Kühl A,Loddenkemper C,Witzenrath M,Kube S,Henke W,Hamelmann E

14-7042 was used in Immunohistochemistry to show that targeting STAT6 by siRNA effectively blocks the development of cardinal features of allergic airway disease.

Mon Jun 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

Gene Aliases: BSF-1; Il-4; Il4

UniProt ID: (Mouse) P07750

Entrez Gene ID: (Mouse) 16189

Molecular Function: interleukin superfamily

Function(s)

Process(es)

cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity

IL-4 Monoclonal Antibody (BVD6-24G2), eBioscience™

Product Details

14-7042-85

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (FN)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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