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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Recombinant mouse IL-5|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Inhibition Assays (IA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM550D targets IL-5 in ELISA, IA, and WB applications and shows reactivity with mouse samples.
The MM550D immunogen is recombinant mouse IL-5.
MM550D detects IL-5 which has a predicted molecular weight of approximately 13 kDa.
The MM550D mouse IL5 antibody (clone TRFK4) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM550CB (biotinylated conjugate of clone TRFK5). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody MM550D (clone TRFK4) and biotinylated antibody MM550CB (clone TRFK5) have successfully been used in combination with recombinant mouse IL5 protein SMIL5 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This product is a Low Endotoxin formulation.
The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Chronic intestinal nematode infection induces Stat6-independent interleukin-5 production and causes eosinophilic inflammatory responses in mice.
MM550D was used in ELISA to investigate the role of STAT6 for the immune response induced by Nippostrongylus brasiliensis
|Sakamoto Y,Hiromatsu K,Ishiwata K,Inagaki-Ohara K,Ikeda T,Nakamura-Uchiyama F,Nawa Y||Immunology (112:615)||2004|
Vaccination with recombinant alphavirus or immune-stimulating complex antigen against respiratory syncytial virus.
MM550D was used in ELISA to study the vaccination against respiratory syncytial virus
|Chen M,Hu KF,Rozell B,Orvell C,Morein B,Liljeström P||Journal of immunology (Baltimore, Md. : 1950) (169:3208)||2002|
Effects of birch pollen and traffic particulate matter on Th2 cytokines, immunoglobulin E levels and bronchial hyper-responsiveness in mice.
MM550D was used in ELISA to study the role of pollen and traffic particulate matter in the initiation and persistence of experimental allergic inflammation
|Fernvik E,Peltre G,Sénéchal H,Vargaftig BB||Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (32:602)||2002|
Oral administration of L-arginine potentiates allergen-induced airway inflammation and expression of interleukin-5 in mice.
MM550D was used in ELISA to investigate the effect of L-arginine administration on airway inflammation and cytokine expression
|Takano H,Lim HB,Miyabara Y,Ichinose T,Yoshikawa T,Sagai M||The Journal of pharmacology and experimental therapeutics (286:767)||1998|
Long-term exposure to diesel exhaust enhances antigen-induced eosinophilic inflammation and epithelial damage in the murine airway.
MM550D was used in ELISA to investigate the effect of diesel exhaust on the damage of mouse airway system
|Ichinose T,Takano H,Miyabara Y,Sagai M||Toxicological sciences : an official journal of the Society of Toxicology (44:70)||1998|
Murine strain differences in airway inflammation caused by diesel exhaust particles.
MM550D was used in ELISA to investigate the strain-specific inflammatory responses against air pollutants
|Miyabara Y,Yanagisawa R,Shimojo N,Takano H,Lim HB,Ichinose T,Sagai M||The European respiratory journal (11:291)||1998|
Diesel exhaust particles enhance antigen-induced airway inflammation and local cytokine expression in mice.
MM550D was used in ELISA to investigate the effect of diesel exhaust particles on airway inflammation and cytokine expression
|Takano H,Yoshikawa T,Ichinose T,Miyabara Y,Imaoka K,Sagai M||American journal of respiratory and critical care medicine (156:36)||1997|
Synthesis of T helper 2-type cytokines at the maternal-fetal interface.
MM550D was used in ELISA to investigate the expression of Th2 cytokines at the maternal-fetal interface
|Lin H,Mosmann TR,Guilbert L,Tuntipopipat S,Wegmann TG||Journal of immunology (Baltimore, Md. : 1950) (151:4562)||1993|
Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.
MM550D was used in blocking/activating experiment to investigate the effect of CD4 antibodies on Th1 differentiation
|Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E||Journal of immunology (Baltimore, Md. : 1950) (157:566)||1996|
Delineation of IL-5 domains predicted to engage the IL-5 receptor complex.
MM550D was used in blocking/activating experiment to study the mechanism for the effect of interleukin 5
|Dickason RR,Huston MM,Huston DP||Journal of immunology (Baltimore, Md. : 1950) (156:1030)||1996|
B-cell growth factor II; BCGF-II; cytotoxic T-lymphocyte inducer; eosinophil differentiation factor; interleukin-5; T-cell replacing factor; TRF