|Tested species reactivity||Mouse|
|Published species reactivity||Hamster, Mouse|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant mouse IL-5|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM550CB targets IL-5 in ELISA, and WB applications and shows reactivity with mouse samples.
The MM550CB immunogen is recombinant mouse IL-5.
MM550CB detects IL-5 which has a predicted molecular weight of approximately 13 kDa.
The MM550CB mouse IL5 antibody (biotinylated conjugate of clone TRFK5) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM550D (clone TRFK4). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody MM550CB (clone TRFK5) and antibody MM550D (clone TRFK4) have successfully been used in combination with recombinant mouse IL5 protein SMIL5 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Chronic intestinal nematode infection induces Stat6-independent interleukin-5 production and causes eosinophilic inflammatory responses in mice.
MM550CB was used in ELISA to investigate the role of STAT6 for the immune response induced by Nippostrongylus brasiliensis
|Sakamoto Y,Hiromatsu K,Ishiwata K,Inagaki-Ohara K,Ikeda T,Nakamura-Uchiyama F,Nawa Y||Immunology (112:615)||2004|
Vaccination with recombinant alphavirus or immune-stimulating complex antigen against respiratory syncytial virus.
MM550CB was used in ELISA to study the vaccination against respiratory syncytial virus
|Chen M,Hu KF,Rozell B,Orvell C,Morein B,Liljeström P||Journal of immunology (Baltimore, Md. : 1950) (169:3208)||2002|
Effects of birch pollen and traffic particulate matter on Th2 cytokines, immunoglobulin E levels and bronchial hyper-responsiveness in mice.
MM550CB was used in ELISA to study the role of pollen and traffic particulate matter in the initiation and persistence of experimental allergic inflammation
|Fernvik E,Peltre G,Sénéchal H,Vargaftig BB||Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (32:602)||2002|
Oral administration of L-arginine potentiates allergen-induced airway inflammation and expression of interleukin-5 in mice.
MM550CB was used in ELISA to investigate the effect of L-arginine administration on airway inflammation and cytokine expression
|Takano H,Lim HB,Miyabara Y,Ichinose T,Yoshikawa T,Sagai M||The Journal of pharmacology and experimental therapeutics (286:767)||1998|
Long-term exposure to diesel exhaust enhances antigen-induced eosinophilic inflammation and epithelial damage in the murine airway.
MM550CB was used in ELISA to investigate the effect of diesel exhaust on the damage of mouse airway system
|Ichinose T,Takano H,Miyabara Y,Sagai M||Toxicological sciences : an official journal of the Society of Toxicology (44:70)||1998|
IL-5-dependent immunity to microfilariae is independent of IL-4 in a mouse model of onchocerciasis.
MM550CB was used in ELISA to study the roles of cytokines in helminth immune responses
|Hogarth PJ,Taylor MJ,Bianco AE||Journal of immunology (Baltimore, Md. : 1950) (160:5436)||1998|
Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections.
MM550CB was used in ELISA to investigate the effect of phosphatidylcholine immunization of mice for the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections
|Bordmann G,Rudin W,Favre N||Immunology (94:35)||1998|
Murine strain differences in airway inflammation caused by diesel exhaust particles.
MM550CB was used in ELISA to investigate the strain-specific inflammatory responses against air pollutants
|Miyabara Y,Yanagisawa R,Shimojo N,Takano H,Lim HB,Ichinose T,Sagai M||The European respiratory journal (11:291)||1998|
Diesel exhaust particles enhance antigen-induced airway inflammation and local cytokine expression in mice.
MM550CB was used in ELISA to investigate the effect of diesel exhaust particles on airway inflammation and cytokine expression
|Takano H,Yoshikawa T,Ichinose T,Miyabara Y,Imaoka K,Sagai M||American journal of respiratory and critical care medicine (156:36)||1997|
Allospecific CD8+ Tc1 and Tc2 populations in graft-versus-leukemia effect and graft-versus-host disease.
MM550CB was used in ELISA to study ways to improve allogeneic T cell transfers in leukemia patients
|Fowler DH,Breglio J,Nagel G,Eckhaus MA,Gress RE||Journal of immunology (Baltimore, Md. : 1950) (157:4811)||1996|
Adjuvant-free hsp70 fusion protein system elicits humoral and cellular immune responses to HIV-1 p24.
MM550CB was used in ELISA to investigate the immunogenicity of Mycobacterium tuberculosis heat shock proteins
|Suzue K,Young RA||Journal of immunology (Baltimore, Md. : 1950) (156:873)||1996|
IL-12-induced protection against blood-stage Plasmodium chabaudi AS requires IFN-gamma and TNF-alpha and occurs via a nitric oxide-dependent mechanism.
MM550CB was used in ELISA to study the mechanism for the effect of interleukin 12 on Plasmodium chabaudi
|Stevenson MM,Tam MF,Wolf SF,Sher A||Journal of immunology (Baltimore, Md. : 1950) (155:2545)||1995|
Synthesis of T helper 2-type cytokines at the maternal-fetal interface.
MM550CB was used in ELISA to investigate the expression of Th2 cytokines at the maternal-fetal interface
|Lin H,Mosmann TR,Guilbert L,Tuntipopipat S,Wegmann TG||Journal of immunology (Baltimore, Md. : 1950) (151:4562)||1993|
Depletion of eosinophil infiltration by anti-IL-5 monoclonal antibody (TRFK-5) accelerates open skin wound epithelial closure.
MM550CB was used in blocking/activating experiment to study the effect of eosinophil depletion on wound healing
|Yang J,Torio A,Donoff RB,Gallagher GT,Egan R,Weller PF,Wong DT||The American journal of pathology (151:813)||1997|