|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Purified, recombinant human interleukin 5 soluble receptor alpha (IL5RA) expressed in insect Sf 21 cells.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Flow Cytometry (Flow)||50-250 ng for 10^6 cells|
|Inhibition Assays (IA)||1 - 3 µg/ml|
|Neutralization (Neu)||1-3 µg/ml|
|Western Blot (WB)||0.1-0.2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24792 detects IL5RA from human samples.
PA1-24792 has been successfully used in Western blot, ELISA, Flow cytometry, Neutralization and Inhibition assay procedures. The Neutralization Dose50 (ND50) for anti-human IL5RA is approximately 1-3 ug/ml in the presence of 0.5 ng/ml of recombinant human IL-5 using the human TF-1 cell line. The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cell surface IL5RA mediated IL5 response on a responsive cell line, at a specific IL5 concentration. The exact concentration of antibody required to neutralize the human cell surface IL5RA mediated bioactivity is dependent on the IL5 concentration as well as on the number of IL5RA present on the cell surface (a function of the cell type and culture conditions).
The PA1-24792 immunogen is purified, recombinant human interleukin 5 soluble receptor alpha (IL5RA) expressed in insect Sf 21 cells.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.