Sandwich ELISA analysis of mouse IL-6 was performed using the a Mouse IL-6 Colorimetric ELISA Kit (Product # EM2IL6) by loading 50 ul per well of Mouse IL-6 Recombinant Protein (Product # SMIL6) in dodecuplicate at 2000, 800, 320, 128, 51, and 0 pg/ml across a 3 ug/ml IL-6 monoclonal antibody (Product # MM600D) pre-coated plate and incubating for 2 hours at room temperature. The plate was washed, then incubated with 50 ul per well of a biotinylated-anti-IL-6 antibody (Product # MM600CB) in dodecuplicate at 0.25 ug/ml for 1 hour at room temperature. The plate was washed and incubated with 100 ul per well of Streptavidin-HRP (Product # N504) at a dilution of 1:100,000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB-ELISA substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Recombinant mouse IL-6|
|Purification||Ammonium sulfate precipitation|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM600D targets IL-6 in ELISA, and WB applications and shows reactivity with mouse samples.
The MM600D immunogen is recombinant mouse IL-6.
MM600D detects IL-6 which has a predicted molecular weight of approximately 22 kDa.
The MM600D mouse IL6 antibody (clone 32C11) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM600CB (biotinylated conjugate of clone 20F3). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody MM600D (clone 32C11) and biotinylated antibody MM600CB (clone 20F3) have successfully been used in combination with recombinant mouse IL6 protein SMIL6 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PmpG303-311, a protective vaccine epitope that elicits persistent cellular immune responses in Chlamydia muridarum-immune mice.
MM600D was used in ELISA to study a protective vaccine epitope that elicits persistent cellular immune responses in Chlamydia muridarum-immune mice
|Johnson RM,Yu H,Kerr MS,Slaven JE,Karunakaran KP,Brunham RC||Infection and immunity (80:2204)||2012|
Real-time imaging of mechanically injured femoral artery in mice reveals a biphasic pattern of leukocyte accumulation.
MM600D was used in ELISA to investigate the leukocyte accumulation process during artery injury
|Osaka M,Hagita S,Haraguchi M,Kajimura M,Suematsu M,Yoshida M||American journal of physiology. Heart and circulatory physiology (292:H1876)||2007|
Pattern recognition molecules activated by Chlamydia muridarum infection of cloned murine oviduct epithelial cell lines.
MM600D was used in ELISA to investigate the role of TLRs during the responses of epithelial cells induced by C. trachomatis infection
|Derbigny WA,Kerr MS,Johnson RM||Journal of immunology (Baltimore, Md. : 1950) (175:6065)||2005|
Inhibition of vagally mediated immune-to-brain signaling by vanadyl sulfate speeds recovery from sickness.
MM600D was used in ELISA to investigate the role of TLR2 in the interferon gamma responses
|Johnson DR,O'Connor JC,Dantzer R,Freund GG||Proceedings of the National Academy of Sciences of the United States of America (102:15184)||2005|
Anti-IL-6 monoclonal antibodies protect against lethal Escherichia coli infection and lethal tumor necrosis factor-alpha challenge in mice.
MM600D was used in blocking/activating experiment to study the effect of interleukin 6 antibodies on Escherichia coli infection and tumor necrosis factor alpha challenge in mice
|Starnes HF,Pearce M,Yim J,Abrams J,Tewari A,Zou J||Journal of immunology (Baltimore, Md. : 1950) (148:null)||1992|