Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.1-0.2 µg/mL | - |
ELISA (ELISA) |
0.25-1.0 µg/mL | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
E.coli-derived Recombinant Human IL-6 |
Conjugate |
Biotin |
Form |
Lyophilized |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS |
Contains |
no preservative |
Storage conditions |
-20°C |
AA Sequence of recombinant protein: PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-6. Anti-Human IL-6-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human IL-6 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-6 (500-P26) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-6.
Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6
Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6
UniProt ID: (Human) P05231
Entrez Gene ID: (Human) 3569
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