Invitrogen

IL-6 Monoclonal Antibody (MP5-20F3), PE, eBioscience™

Product Image Gallery

IL-6 Antibody in Flow Cytometry (Flow) — Mouse thioglycolate-induced peritoneal exudate cells were stimulated with LPS in the presence of Brefeldin A for 6 hours (right) or unstimulated (left). The cells were surface stained with Anti-Mouse F4/80 Antigen FITC (Product # 11-4801-82) and intracellularly stained with Anti-Mouse IL-6 PE.

Product Details

12-7061-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.25 µg/test	View 16 publications 16 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	MP5-20F3
Conjugate	PE eFluor 450 FITC Functional Grade PerCP-eFluor 710 Unconjugated
Excitation/Emission Max	565/576 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_466165

Product Specific Information

Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

Applications Reported: This MP5-20F3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MP5-20F3 antibody is offered in 2 formats: - µg size: has been tested by intracellular staining and flow cytometric analysis of stimulated peritoneal macrophages. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated peritoneal macrophages. This can be used at test size: 5 µL (0.125 µg) per test.The pre-titrated test size contains BSA and/or gelatin for protein stabilization.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

Published figure using IL-6 monoclonal antibody (Product # 12-7061-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

Published figure using IL-6 monoclonal antibody (Product # 12-7061-82) in Flow Cytometry

Wed Feb 16 00:00:00 EST 2022

Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver.

Immunity

Remmerie A,Martens L,Thoné T,Castoldi A,Seurinck R,Pavie B,Roels J,Vanneste B,De Prijck S,Vanhockerhout M,Binte Abdul Latib M,Devisscher L,Hoorens A,Bonnardel J,Vandamme N,Kremer A,Borghgraef P,Van Vlierberghe H,Lippens S,Pearce E,Saeys Y,Scott CL

12-7061 was used in Flow cytometry/Cell sorting to highlight considerable heterogeneity within the macrophage pool and suggest a need for more specific macrophage targeting strategies in metabolic-associated fatty liver disease (MAFLD).

Tue Sep 15 00:00:00 EDT 2020

STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice.

JCI insight

Cai W,Dai X,Chen J,Zhao J,Xu M,Zhang L,Yang B,Zhang W,Rocha M,Nakao T,Kofler J,Shi Y,Stetler RA,Hu X,Chen J

12-7061 was used in Flow cytometry/Cell sorting to suggest that efferocytosis via STAT6/Arg1 modulates microglia/macrophage phenotype, accelerates inflammation resolution, and improves stroke outcomes.

Thu Oct 17 00:00:00 EDT 2019

Protective role of follicular CXCR5⁺CD8⁺ T cells against dengue virus 2 infection.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

Qiu L,Wang H,Yu Q,Liu J,Chen S,Zhao Z

12-7061-82 was used in Flow Cytometry to show that CXCR5+CD8+ T cells could play a protective role in dengue pathogenesis and may be a novel strategy for controlling DENV2 infection and vaccine development.

Sat Jun 01 00:00:00 EDT 2019

1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.

JCI insight

Wasnik S,Rundle CH,Baylink DJ,Yazdi MS,Carreon EE,Xu Y,Qin X,Lau KW,Tang X

Published figure using IL-6 monoclonal antibody (Product # 12-7061-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

Loss of NF-κB1 Causes Gastric Cancer with Aberrant Inflammation and Expression of Immune Checkpoint Regulators in a STAT-1-Dependent Manner.

Immunity

O'Reilly LA,Putoczki TL,Mielke LA,Low JT,Lin A,Preaudet A,Herold MJ,Yaprianto K,Tai L,Kueh A,Pacini G,Ferrero RL,Gugasyan R,Hu Y,Christie M,Wilcox S,Grumont R,Griffin MDW,O'Connor L,Smyth GK,Ernst M,Waring P,Gerondakis S,Strasser A

12-7061-82 was used in Flow Cytometry to provide mechanistic insight into how polymorphisms that attenuate NFKB1 expression predispose humans to epithelial cancers, highlighting the pro-tumorigenic activity of STAT1 and identifying targetable vulnerabilities in gastric cancer.

Tue Mar 20 00:00:00 EDT 2018

Identification of 14-dehydroergosterol as a novel anti-inflammatory compound inducing tolerogenic dendritic cells.

Scientific reports

Ano Y,Ikado K,Shindo K,Koizumi H,Fujiwara D

Tue Oct 24 00:00:00 EDT 2017

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

Thu Jun 01 00:00:00 EDT 2017

IL-6 Regulates M2 Polarization and Local Proliferation of Adipose Tissue Macrophages in Obesity.

Journal of immunology (Baltimore, Md. : 1950)

Braune J,Weyer U,Hobusch C,Mauer J,Brüning JC,Bechmann I,Gericke M

12-7061 was used in Flow cytometry/Cell sorting to study how IL-6 acts as a Th2 cytokine in obesity by stimulating M2 polarisation and local adipose tissue macrophages proliferation.

Sat Apr 01 00:00:00 EDT 2017

Intestinal barrier dysfunction develops at the onset of experimental autoimmune encephalomyelitis, and can be induced by adoptive transfer of auto-reactive T cells.

PloS one

Nouri M,Bredberg A,Weström B,Lavasani S

12-7061 was used in Flow cytometry/Cell sorting to show that disruption of intestinal homeostasis is an early and immune-mediated event in experimental autoimmune encephalomyelitis.

Thu May 14 00:00:00 EDT 2015

Tumor suppressor p53 inhibits systemic autoimmune diseases by inducing regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Kawashima H,Takatori H,Suzuki K,Iwata A,Yokota M,Suto A,Minamino T,Hirose K,Nakajima H

12-7061 was used in Flow cytometry/Cell sorting to investigate how p53 suppresses autoimmunity.

Tue Oct 01 00:00:00 EDT 2013

Parvovirus evades interferon-dependent viral control in primary mouse embryonic fibroblasts.

Virology

Mattei LM,Cotmore SF,Tattersall P,Iwasaki A

12-7061 was used in Flow cytometry/Cell sorting to suggest that minute virus of mice efficiently evades antiviral immune mechanisms of type I IFNs, explaining their efficient transmission between mice.

Sat Jul 20 00:00:00 EDT 2013

CD8 regulates T regulatory cell production of IL-6 and maintains their suppressive phenotype in allergic lung disease.

Journal of immunology (Baltimore, Md. : 1950)

Joetham A,Okamoto M,Takeda K,Schedel M,Ohnishi H,Dakhama A,Gelfand EW

12-7061 was used in Flow cytometry/Cell sorting to identify the critical role of CD8-T regulatory cell interactions in regulating the suppressive phenotype of nTregs through control of IL-6 production.

Sat Jan 01 00:00:00 EST 2011

Mechanisms by which chronic ethanol feeding limits the ability of dendritic cells to stimulate T-cell proliferation.

Alcoholism, clinical and experimental research

Fan J,Edsen-Moore MR,Turner LE,Cook RT,Legge KL,Waldschmidt TJ,Schlueter AJ

12-7061 was used in Flow cytometry/Cell sorting to assess the functional capability of ethanol-exposed dendritic cells, using a murine model of chronic alcohol abuse.

Sat Jan 01 00:00:00 EST 2011

Mast cells counteract regulatory T-cell suppression through interleukin-6 and OX40/OX40L axis toward Th17-cell differentiation.

Blood

Piconese S,Gri G,Tripodo C,Musio S,Gorzanelli A,Frossi B,Pedotti R,Pucillo CE,Colombo MP

12-7061 was used in Flow cytometry/Cell sorting to show that mast cells, an early source of inflammatory mediators, are able to counteract Treg inhibition over effector T cells.

Thu Sep 24 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

Gene Aliases: Il-6; Il6

UniProt ID: (Mouse) P08505

Entrez Gene ID: (Mouse) 16193

Function(s)

Process(es)

receptor binding cytokine activity interleukin-6 receptor binding protein binding growth factor activity

IL-6 Monoclonal Antibody (MP5-20F3), PE, eBioscience™

Product Details

12-7061-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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