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IL-6 Monoclonal Antibody (MP5-20F3), eBioscience™

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IL-6 Antibody (14-7061-81) in IS

Mouse splenocytes (500,000 cells/ well) were activated with LPS (1 µg/mL) for 48 hours in Mouse IL-6 ELISPOT assay. Control well is medium alone.

Product Image Gallery

IL-6 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Sox2 overexpression suppressed LPS-induced upregulation of IL6 and TNF-alpha. A IL6 immunofluorescence in cochlear cross-section after LPS treatment. Myosin7a is used as a marker of cochlear HCs. (B) Western blot analysis of sensory epithelia shows increased expression levels of TNF-alpha and IL6 in control mice after LPS treatment, but the LPS-induced increase of the TNF-alpha and IL6 protein was significantly inhibited in Prestin-Sox2OE mice. beta-Actin served as the sample loading control. Data are presented as means +- SEM. ; n = 3. * P < 0.05, ** P < 0.01. Scale bar = 20 mum

IL-6 Antibody in Immunocytochemistry (ICC/IF) — Figure 3 Effect of betulinic acid (BA) on production of proinflammatory cytokines. ( A ) Pancreatic mRNA levels of interleukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). ( B ) Pancreatic protein levels of IL-1beta, IL-6, and TNF-alpha were measured by ELISA. ( C ) Confocal images of immunofluorescence staining of IL-1beta (red), IL-6 (green), TNF-alpha (red), and DAPI (blue). Scale bar = 20 mum. ( D ) Pancreatic acinar cells (PACs) were pretreated with BA (10 uM) for 1 h and then stimulated with cerulein (10 nM) for 6 h. The cell viability was measured as described in the experimental protocol. ( E ) PACs were pretreated with BA for 1 h at the indicated dose, and then stimulated with cerulein (10 nM) for 24 h. mRNA levels of IL-1beta, IL-6, and TNF-alpha in PACs were determined using RT-qPCR. Data have been represented as mean +- SEM for six mice in each group. Results are representative of three experiments. * p < 0.05 vs. dimethyl sulfoxide (DMSO) treatment alone. + p < 0.05 vs. cerulein treatment alone.

IL-6 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Sox2 overexpression suppressed noise-induced upregulation of IL6 and TNF-alpha. A IL6 immunofluorescence in cochlear cross-section after noise treatment. The IL6 expression level was elevated after traumatic noise exposure in HCs, while it was inhibited in the Sox2 overexpression group. Myosin7a is used as a marker of cochlear HCs. B Western blot analysis of sensory epithelia shows increased expression levels of TNF-alpha and IL6 in control mice after noise injury, but it was significantly inhibited in Prestin-Sox2OE mice. beta-Actin served as the sample loading control. Data are presented as means +- SEM. ; n = 3. * P < 0.05, ** P < 0.01. Scale bar = 20 mum

IL-6 Antibody in Immunohistochemistry (IHC) — Sox2 upregulation mitigated inflammation-induced HC loss. A Experimental protocol for the LPS-induced HC loss model. B Myosin7a immunofluorescence in cochlear HCs of adult PrestinCreER +/- /Sox2OE +/- mice and control mice with or without LPS treatment. C Cell counting results of cochlear HCs after 4 mg/kg of LPS administration. D TNF-alpha immunofluorescence in cochlear cross-section after LPS challenge. E The mRNA expression levels of pro-inflammatory cytokines. Sox2 overexpression prevented the LPS-induced increase in pro-inflammatory cytokines. Data are presented as means +- SEM; n = 6; ** P < 0.01, and *** P < 0.001. Scale bar = 20 mum

IL-6 Antibody in Immunohistochemistry, ELISA (IHC, ELISA) — Fig. 4 Expression of IL-6 in the urinary bladder tissue compartments upon UPEC infection. a , b Mice were infected with UPEC and analyzed 1-day post-infection. a Representative spatial distribution of IL-6 in healthy (left) and UPEC-infected (right) bladders by MALDI-MSI. The top row shows a greyscale image and the tissue segmentation of the urothelium from the urinary bladder. The bottom row demonstrates the expression of IL-6. The white lines separate the connective tissue from the urothelium and the lumen. The segmented urothelium and lumen are represented as individual images on the far right and far left sides. b Within the urothelium, the average intensity of IL-6 per pixel per sample was collected. c Longitudinal study on the concentration of IL-6 in bladder homogenates by ELISA (0 h: n = 6, 1 h: n = 6, 2 h: n = 6, 24 h: n = 5). d Detection of IL-6 on cryosections 3 h after infection by immunofluorescence microscopy. Scale bar indicates 50 um and dashed lines separate the urothelium from the connective tissue. ** p < 0.01. Error bars show the mean +- SEM. con, connective tissue; uro, urothelium.

IL-6 Antibody in Western Blot (WB) — Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Western Blot

IL-6 Antibody in Flow Cytometry (Flow) — Decreased exosomal let-7s are responsible for immunosuppressive niche establishment. a Let-7s and miR-125s family members were specially reduced in the circulation exosomes in mice bearing 4TO7-Lin28B tumor ( n = 3 mice in each group). b The experiment was the same as in panel a , except that let-7s and miR-125s family members were measured in the serum exosomes of PyMT mice (11-week old) ( n = 3 mice in each group). c Let-7a inhibited increased production of IL-6 in neutrophils mediated by Lin28B-exosome. Naive mice were i.v injected with Control-exosome or Lin28B-exosome in the presence of the indicated agomir. IL-6 expression was determined in lung neutrophils by FACS ( n = 6 mice in each group). d The experiment was the same as for panel c , except that the IL-10 was detected in macrophages ( n = 6 mice in each group). e Let-7a inhibited neutrophil N2 conversion induced by Lin28B-exosomes. The exosome treatment was the same as for panel c. GAPDH served as an internal control ( n = 6 mice in each group). f Let-7a rescued T cell inactivation induced by Lin28B-exosome ( n = 6 mice in each group). g Let-7a restored Lin28B-exosome-mediated inhibitory Th1 differentiation of CD4 + T cells ( n = 6 mice in each group). h Let-7a attenuated Lin28B-exosome-mediated lung metastasis ( n = 6 mice in each group). i ALDH + and ALDH - subsets of 4TO7-Lin28B cells isolated from primary tumors were analyzed for exosomal miRNA expression. U6 served as an internal control ( n = 3 mice in each

IL-6 Antibody in ELISA (ELISA) — Fig. 6 5-MTP suppresses HFD-induced IL-6 and HSP60 in the atherosclerotic lesions of ApoE -/- mice. Chow diet-fed or HFD-fed ApoE -/- mice were treated with saline or L-5-MTP twice weekly for 20 wk. ( A , B ) IL-6 and ( C , D ) OPG in paraffin-embedded aortic tissues were analyzed by immunohistochemistry. Negative control represents staining with a 2nd antibody. Representative microphotographs of the immunohistochemical staining for ( A ) IL-6 and ( C ) OPG are shown. Immunopositive areas of ( B ) IL-6 and ( D ) OPG in aorta tissues were quantified as a percentage of the total aortic area in each section. Data represent mean +- SEM (Chow + saline group: n = 6, HFD + saline group: n = 6 and HFD + 5-MTP group: n = 5). E Plasma IL-6 levels in HFD-fed ApoE -/- mice with injection of saline (n = 19) or L-5-MTP (n = 9) twice weekly for 20 wk for were measured by ELISA. Data represent mean +- SEM. After pretreating VSMCs with DMSO, DL-5-MTP, or L-5-MTP (100 muM) for 30 min, VSMCs were treated with vehicle or Pam3CSK4 (Pam3) in calcifying medium for 24 h. F IL6 and G OPG level in culture supernatants was measured by ELISA. Data represent mean +- SD of at least 3 independent experiments. HSP60 contents in aorta tissues from ApoE -/- mice fed chow diet with saline (n = 5) or HFD with saline (n = 5) or L-5-MTP (n = 5) for 20 wk were analyzed by immunohistochemistry. Representative microphotographs of the immunohistochemical staining ( H ) and quantification of immunopositive positive ar

IL-6 Antibody in Neutralization (Neu) — Figure 2. Deletion of IL-6 from KPC cells prevented muscle wasting in vitro and increased survival in mice. (A) Targeted mutagenesis of the Il6 gene was performed, and a transfection control clone (KPC) and an Il6 ablated clone (KPC IL6 KO ) were selected for use in downstream experiments based on their Il6 expression relative to the untransfected parental cell line (KPC-P). Clones were cultured individually in triplicate for n = 3 per group, and error bars are mean plus SD. (B) To determine if deletion of IL-6 affected tumor cell growth, a proliferation assay was performed comparing the clones, where clones were cultured in triplicate ( n = 3 per group) and measurements were taken every hour for 100 h; growth curves represent the mean proliferation of triplicate wells over time. (C) Myoblasts were differentiated into myotubes and treated in triplicate ( n = 3 per group) with 30% CM from tumor clones to measure effects on myotube atrophy and the activation of STAT3. (D) Western blotting (WB) analysis using the pooled myotube protein lysates from n = 3 per group was performed to measure phosphorylation of STAT3 (p-STAT3) as an indication of STAT3 activation. (E and F) Myotubes were visualized using IF with an anti-myosin heavy chain (MHC) antibody (E), and myotube atrophy was measured from 20 random fields per well (n~150 myotube diameter measurements per well, n = 3 wells per group). Scale bar = 50 um. Error bars represent mean myotube diameter and SD, and significant differe

Product Details

14-7061-81

Applications

Tested Dilution

Publications

Western Blot (WB)

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 15 publications 15 publications

ELISA (ELISA)

View 20 publications 20 publications

Neutralization (Neu)

View 4 publications 4 publications

Functional Assay (FN)

View 3 publications 3 publications

Blocking Assay (BLOCK)

View 1 publication 1 publication

Immunostaining (IS)

0.5-2 µg/mL

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MP5-20F3

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_468422

Product Specific Information

Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

Applications Reported: This MP5-20F3 antibody has been reported for use in ELISA capture antibody and ELISPOT capture antibody.

Applications Tested: The MP5-20F3 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-6 (IL-6) in combination with the biotin MP5-32C11 (cat. 13-7062) antibody for detection and recombinant mouse IL-6 (cat. 14-8061) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 8-1000 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Flow Cytometry

Wed Feb 16 00:00:00 EST 2022

Sox2 overexpression alleviates noise-induced hearing loss by inhibiting inflammation-related hair cell apoptosis.

Journal of neuroinflammation

Chen D,Jia G,Zhang Y,Mao H,Zhao L,Li W,Chen Y,Ni Y

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Immunocytochemistry

Mon Feb 28 00:00:00 EST 2022

Sox2 overexpression alleviates noise-induced hearing loss by inhibiting inflammation-related hair cell apoptosis.

Journal of neuroinflammation

Chen D,Jia G,Zhang Y,Mao H,Zhao L,Li W,Chen Y,Ni Y

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Immunocytochemistry

Mon Feb 28 00:00:00 EST 2022

Sox2 overexpression alleviates noise-induced hearing loss by inhibiting inflammation-related hair cell apoptosis.

Journal of neuroinflammation

Chen D,Jia G,Zhang Y,Mao H,Zhao L,Li W,Chen Y,Ni Y

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Immunocytochemistry

Mon Feb 28 00:00:00 EST 2022

Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.

Immunity

Xian H,Liu Y,Rundberg Nilsson A,Gatchalian R,Crother TR,Tourtellotte WG,Zhang Y,Aleman-Muench GR,Lewis G,Chen W,Kang S,Luevanos M,Trudler D,Lipton SA,Soroosh P,Teijaro J,de la Torre JC,Arditi M,Karin M,Sanchez-Lopez E

14-7061-81 was used in ELISA to conclude that inhibition of ATP and mtDNA synthesis is sufficient for ARDS amelioration.

Tue Jul 13 00:00:00 EDT 2021

Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.

Immunity

14-7061-81 was used in ELISA to conclude that inhibition of ATP and mtDNA synthesis is sufficient for ARDS amelioration.

Tue Jul 13 00:00:00 EDT 2021

Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexia.

The Journal of experimental medicine

Rupert JE,Narasimhan A,Jengelley DHA,Jiang Y,Liu J,Au E,Silverman LM,Sandusky G,Bonetto A,Cao S,Lu X,O'Connell TM,Liu Y,Koniaris LG,Zimmers TA

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Neutralization

Mon Jun 07 00:00:00 EDT 2021

Restoration of 5-methoxytryptophan protects against atherosclerotic chondrogenesis and calcification in ApoE^-/- mice fed high fat diet.

Journal of biomedical science

Lee GL,Liao TL,Wu JY,Wu KK,Kuo CC

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in ELISA

Mon Nov 08 00:00:00 EST 2021

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in ELISA

Fri Jul 02 00:00:00 EDT 2021

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in ELISA

Fri Jul 02 00:00:00 EDT 2021

Betulinic Acid Ameliorates the Severity of Acute Pancreatitis via Inhibition of the NF-κB Signaling Pathway in Mice.

International journal of molecular sciences

Zhou Z,Choi JW,Shin JY,Kim DU,Kweon B,Oh H,Kim YC,Song HJ,Bae GS,Park SJ

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Immunocytochemistry

Sat Jun 26 00:00:00 EDT 2021

Origin and Function of Stress-Induced IL-6 in Murine Models.

Cell

Qing H,Desrouleaux R,Israni-Winger K,Mineur YS,Fogelman N,Zhang C,Rashed S,Palm NW,Sinha R,Picciotto MR,Perry RJ,Wang A

14-7061-81 was used in Immunohistochemistry to study the cytokine interleukin-6 (IL-6) and its induction following acute stress in mouse models.

Thu Jul 23 00:00:00 EDT 2020

Origin and Function of Stress-Induced IL-6 in Murine Models.

Cell

Qing H,Desrouleaux R,Israni-Winger K,Mineur YS,Fogelman N,Zhang C,Rashed S,Palm NW,Sinha R,Picciotto MR,Perry RJ,Wang A

14-7061-81 was used in Immunohistochemistry to study the cytokine interleukin-6 (IL-6) and its induction following acute stress in mouse models.

Thu Jul 23 00:00:00 EDT 2020

Spatial proteomics revealed a CX₃CL1-dependent crosstalk between the urothelium and relocated macrophages through IL-6 during an acute bacterial infection in the urinary bladder.

Mucosal immunology

Bottek J,Soun C,Lill JK,Dixit A,Thiebes S,Beerlage AL,Horstmann M,Urbanek A,Heuer H,Uszkoreit J,Eisenacher M,Bracht T,Sitek B,Hoffmann F,Vijitha N,von Eggeling F,Engel DR

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in ELISA

Wed Jul 01 00:00:00 EDT 2020

Spatial proteomics revealed a CX₃CL1-dependent crosstalk between the urothelium and relocated macrophages through IL-6 during an acute bacterial infection in the urinary bladder.

Mucosal immunology

Bottek J,Soun C,Lill JK,Dixit A,Thiebes S,Beerlage AL,Horstmann M,Urbanek A,Heuer H,Uszkoreit J,Eisenacher M,Bracht T,Sitek B,Hoffmann F,Vijitha N,von Eggeling F,Engel DR

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in ELISA

Wed Jul 01 00:00:00 EDT 2020

Differential Activation of Macrophages Based on Their Environment in Advanced Age.

Chonnam medical journal

Lee DY,Lim JS,Cho KA

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in ELISA

Wed Jan 01 00:00:00 EST 2020

Comprehensive Analysis of the Immune and Stromal Compartments of the CNS in EAE Mice Reveal Pathways by Which Chloroquine Suppresses Neuroinflammation.

Brain sciences

Thome R,Boehm A,Ishikawa LLW,Casella G,Munhoz J,Ciric B,Zhang GX,Rostami A

14-7061-81 was used in Flow Cytometry to analyse the CNS of experimental autoimmune encephalomyelitis mice to identify and characterize the cells that are affected by chloroquine.

Fri Jun 05 00:00:00 EDT 2020

GDF15 Is an Inflammation-Induced Central Mediator of Tissue Tolerance.

Cell

Luan HH,Wang A,Hilliard BK,Carvalho F,Rosen CE,Ahasic AM,Herzog EL,Kang I,Pisani MA,Yu S,Zhang C,Ring AM,Young LH,Medzhitov R

14-7061 was used in an ELISA assay to show that GDF15 coordinates tolerance to inflammatory damage through regulation of triglyceride metabolism.

Thu Aug 22 00:00:00 EDT 2019

Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production.

Cell metabolism

Sanchez-Lopez E,Zhong Z,Stubelius A,Sweeney SR,Booshehri LM,Antonucci L,Liu-Bryan R,Lodi A,Terkeltaub R,Lacal JC,Murphy AN,Hoffman HM,Tiziani S,Guma M,Karin M

14-7061 was used in Western Blotting to conclude that choline kinase inhibitors ameliorated acute and chronic models of IL-1β-dependent inflammation.

Tue Jun 04 00:00:00 EDT 2019

Peli1 controls the survival of dopaminergic neurons through modulating microglia-mediated neuroinflammation.

Scientific reports

Dai D,Yuan J,Wang Y,Xu J,Mao C,Xiao Y

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in ELISA

Wed May 29 00:00:00 EDT 2019

Vanadium Derivative Exposure Promotes Functional Alterations of VSMCs and Consequent Atherosclerosis via ROS/p38/NF-κB-Mediated IL-6 Production.

International journal of molecular sciences

Yeh CC,Wu JY,Lee GL,Wen HT,Lin P,Kuo CC

14-7061-81 was used in ELISA to conclude that NaVO3 exposure activates the ROS-triggering p38 signaling to selectively induce NF-κB-mediated IL-6 production.

Wed Dec 04 00:00:00 EST 2019

Vanadium Derivative Exposure Promotes Functional Alterations of VSMCs and Consequent Atherosclerosis via ROS/p38/NF-κB-Mediated IL-6 Production.

International journal of molecular sciences

Yeh CC,Wu JY,Lee GL,Wen HT,Lin P,Kuo CC

14-7061-81 was used in ELISA to conclude that NaVO3 exposure activates the ROS-triggering p38 signaling to selectively induce NF-κB-mediated IL-6 production.

Wed Dec 04 00:00:00 EST 2019

Serum developmental endothelial locus-1 is associated with severity of sepsis in animals and humans.

Scientific reports

Kim WY,Lee SH,Kim DY,Ryu HJ,Chon GR,Park YY,Fu Y,Huh JW,Lim CM,Koh Y,Choi EY,Hong SB

14-7061-81 was used in ELISA to hypothesize that degradation of the endothelial glycocalyx during sepsis may increase serum Del-1.

Tue Sep 10 00:00:00 EDT 2019

Absence of GdX/UBL4A Protects against Inflammatory Diseases by Regulating NF-кB Signaling in Macrophages and Dendritic Cells.

Theranostics

Liu C,Zhou Y,Li M,Wang Y,Yang L,Yang S,Feng Y,Wang Y,Wang Y,Ren F,Li J,Dong Z,Chin YE,Fu X,Wu L,Chang Z

14-7061-81 was used in ELISA to investigate whether ubiquitin-like protein 4A has a role during the innate immune response.

Fri Jan 31 00:00:00 EST 2020

Frontline Science: Proliferation of Ly6C⁺ monocytes during urinary tract infections is regulated by IL-6 trans-signaling.

Journal of leukocyte biology

Dixit A,Bottek J,Beerlage AL,Schuettpelz J,Thiebes S,Brenzel A,Garbers C,Rose-John S,Mittrücker HW,Squire A,Engel DR

14-7061 was used in Blocking experiments to analyse the proliferative capacity of Ly6C+ monocytes in the urinary bladder after infection with uropathogenic Escherichia coli.

Mon Jan 01 00:00:00 EST 2018

1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.

JCI insight

Wasnik S,Rundle CH,Baylink DJ,Yazdi MS,Carreon EE,Xu Y,Qin X,Lau KW,Tang X

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

Modulation of double-stranded RNA pattern recognition receptor signaling in ovarian cancer cells promotes inflammatory queues.

Oncotarget

Muccioli M,Nandigam H,Loftus T,Singh M,Venkatesh A,Wright J,Pate M,McCall K,Benencia F

14-7061 was used in an ELISA assay to investigate the potential of mouse and human ovarian cancer cells to produce inflammatory factors in response to pathogen recognition receptor signalling.

Fri Nov 30 00:00:00 EST 2018

Increased cathepsin S in Prdm1^-/- dendritic cells alters the T_FH cell repertoire and contributes to lupus.

Nature immunology

Kim SJ,Schätzle S,Ahmed SS,Haap W,Jang SH,Gregersen PK,Georgiou G,Diamond B

14-7061-81 was used in Western Blot to explore the repertoire of follicular helper T cells in patients with lupus.

Fri Sep 01 00:00:00 EDT 2017

Hepatic inflammation caused by dysregulated bile acid synthesis is reversible by butyrate supplementation.

The Journal of pathology

Sheng L,Jena PK,Hu Y,Liu HX,Nagar N,Kalanetra KM,French SW,French SW,Mills DA,Wan YY

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Western Blot

Fri Dec 01 00:00:00 EST 2017

Early IL-6 signalling promotes IL-27 dependent maturation of regulatory T cells in the lungs and resolution of viral immunopathology.

PLoS pathogens

Pyle CJ,Uwadiae FI,Swieboda DP,Harker JA

14-7061-81 was used in Flow Cytometry to show that during respiratory virus infection the prototypic inflammatory cytokine IL-6 is a critical anti-inflammatory regulator of viral induced immunopathology in the respiratory tract through its induction of IL-27.

Fri Sep 01 00:00:00 EDT 2017

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

IL-6 Regulates M2 Polarization and Local Proliferation of Adipose Tissue Macrophages in Obesity.

Journal of immunology (Baltimore, Md. : 1950)

Braune J,Weyer U,Hobusch C,Mauer J,Brüning JC,Bechmann I,Gericke M

14-7061 was used in functional assays to study how IL-6 acts as a Th2 cytokine in obesity by stimulating M2 polarisation and local adipose tissue macrophages proliferation.

Sat Apr 01 00:00:00 EDT 2017

Vitamin A differentially regulates cytokine expression in respiratory epithelial and macrophage cell lines.

Cytokine

Penkert RR,Jones BG,Häcker H,Partridge JF,Hurwitz JL

14-7061 was used in an ELISA assay to evaluate the cell specific effects of retinoic acid on PAMP triggered cytokine expression for LETs and macrophages in mice.

Wed Mar 01 00:00:00 EST 2017

Orally administered sodium 4-phenylbutyrate suppresses the development of dextran sulfate sodium-induced colitis in mice.

Experimental and therapeutic medicine

Ono K,Nimura S,Hideshima Y,Nabeshima K,Nakashima M

14-7061 was used in an ELISA assay to investigate the effects of orally administered sodium 4-phenylbutyrate in mice.

Fri Dec 01 00:00:00 EST 2017

Injury-Induced Senescence Enables In Vivo Reprogramming in Skeletal Muscle.

Cell stem cell

Chiche A,Le Roux I,von Joest M,Sakai H,Aguín SB,Cazin C,Salam R,Fiette L,Alegria O,Flamant P,Tajbakhsh S,Li H

14-7061 was used in Functional assays to study the effects of acute and chronic injury on transcription-factor-mediated reprogramming in skeletal muscle.

Thu Mar 02 00:00:00 EST 2017

Opposing Effects of Fasting Metabolism on Tissue Tolerance in Bacterial and Viral Inflammation.

Cell

Wang A,Huen SC,Luan HH,Yu S,Zhang C,Gallezot JD,Booth CJ,Medzhitov R

14-7061 was used in an ELISA assay to analyse the effects of anorxia in mouse models wih viral and bacterial infections.

Thu Sep 08 00:00:00 EDT 2016

K45A mutation of RIPK1 results in poor necroptosis and cytokine signaling in macrophages, which impacts inflammatory responses in vivo.

Cell death and differentiation

Shutinoski B,Alturki NA,Rijal D,Bertin J,Gough PJ,Schlossmacher MG,Sad S

14-7061 was used in an ELISA assay to investigate the impact of a K45A mutation of RIPK1 on necroptosis of macrophages and the activation of inflammatory response.

Sat Oct 01 00:00:00 EDT 2016

Blockade of MK2 is protective in inflammation-associated colorectal cancer development.

International journal of cancer

Ray AL,Castillo EF,Morris KT,Nofchissey RA,Weston LL,Samedi VG,Hanson JA,Gaestel M,Pinchuk IV,Beswick EJ

14-7061-81 was used in Flow Cytometry to investigate the role of MAPK-activated protein kinase 2 in development of colitis-associated colon cancer.

Mon Feb 01 00:00:00 EST 2016

Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment.

Nature communications

Ulland TK,Jain N,Hornick EE,Elliott EI,Clay GM,Sadler JJ,Mills KA,Janowski AM,Volk AP,Wang K,Legge KL,Gakhar L,Bourdi M,Ferguson PJ,Wilson ME,Cassel SL,Sutterwala FS

14-7061 was used in an ELISA assay to demonstrate that C57BL/6J mice have a functional defect in NLRP12, which is required by macrophages for effective neutrophil recruitment to inflammatory sites.

Tue Oct 25 00:00:00 EDT 2016

Plasmacytoid dendritic cells orchestrate TLR7-mediated innate and adaptive immunity for the initiation of autoimmune inflammation.

Scientific reports

Takagi H,Arimura K,Uto T,Fukaya T,Nakamura T,Choijookhuu N,Hishikawa Y,Sato K

14-7061 was used in Functional assays to investigate the role of plasmacytoid dendritic cells in autoimmunity, showing that they orchestrate TLR7-mediated immune response and initiate autoimmune inflammation.

Thu Apr 14 00:00:00 EDT 2016

γδ T cells promote inflammation and insulin resistance during high fat diet-induced obesity in mice.

Journal of leukocyte biology

Mehta P,Nuotio-Antar AM,Smith CW

14-7061 was used in Flow cytometry/Cell sorting to reveal that isolated γδ T cells directly stimulated RAW264.7 macrophage TNF-α expression but did not stimulate inflammatory mediator expression in 3T3-L1 adipocytes.

Thu Jan 01 00:00:00 EST 2015

Ly6C(high) monocytes control cerebral toxoplasmosis.

Journal of immunology (Baltimore, Md. : 1950)

Biswas A,Bruder D,Wolf SA,Jeron A,Mack M,Heimesaat MM,Dunay IR

14-7061 was used in Flow cytometry/Cell sorting to indicate the critical importance of recruited Ly6C monocytes upon cerebral toxoplasmosis.

Wed Apr 01 00:00:00 EDT 2015

Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation.

PloS one

Pannu J,Belle JI,Förster M,Duerr CU,Shen S,Kane L,Harcourt K,Fritz JH,Clare S,Nijnik A

14-7061 was used in an ELISA assay to indicate that USP21 is redundant for regulation of GATA3 and RIPK1 activity during mouse development, hematopoiesis, and lymphocyte differentiation.

Mon Dec 28 00:00:00 EST 2015

Sprouty1 induces a senescence-associated secretory phenotype by regulating NFκB activity: implications for tumorigenesis.

Cell death and differentiation

Macià A,Vaquero M,Gou-Fàbregas M,Castelblanco E,Valdivielso JM,Anerillas C,Mauricio D,Matias-Guiu X,Ribera J,Encinas M

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Neutralization

Sat Feb 01 00:00:00 EST 2014

Persistently active microbial molecules prolong innate immune tolerance in vivo.

PLoS pathogens

Lu M,Varley AW,Munford RS

14-7061-81 was used in Flow Cytometry to demonstrate a novel mechanism for tolerance reprogramming following exposure to Gram-negative bacteria.

Wed May 01 00:00:00 EDT 2013

Immunoproteasome deficiency modifies the alternative pathway of NFκB signaling.

PloS one

Maldonado M,Kapphahn RJ,Terluk MR,Heuss ND,Yuan C,Gregerson DS,Ferrington DA

14-7061 was used in an ELISA assay to investigate whether the classical or alternative pathways are regulated by the immunoproteasome following chronic TNFα exposure.

Mon Aug 26 00:00:00 EDT 2013

Nicotinic receptor alpha7 expression identifies a novel hematopoietic progenitor lineage.

PloS one

Gahring LC,Enioutina EY,Myers EJ,Spangrude GJ,Efimova OV,Kelley TW,Tvrdik P,Capecchi MR,Rogers SW

14-7061 was used in Flow cytometry/Cell sorting to investigate how inflammatory responses are mechanistically modulated by nicotinic acetylcholine receptors.

Tue Aug 27 00:00:00 EDT 2013

Cytip regulates dendritic-cell function in contact hypersensitivity.

European journal of immunology

Heib V,Sparber F,Tripp CH,Ortner D,Stoitzner P,Heufler C

14-7061 was used in Flow cytometry/Cell sorting to evaluate the functional consequences of cytohesin-interacting protein (Cytip) deficiency in dendritic cells using Cytip knockout mice.

Thu Mar 01 00:00:00 EST 2012

Deletion of Syk in neutrophils prevents immune complex arthritis.

Journal of immunology (Baltimore, Md. : 1950)

Elliott ER,Van Ziffle JA,Scapini P,Sullivan BM,Locksley RM,Lowell CA

14-7061 was used in Flow cytometry/Cell sorting to investigate the specific cell types in which FcγR signalling through spleen tyrosine kinase is required for arthritis development in the serum transfer model.

Sat Oct 15 00:00:00 EDT 2011

Optimal oligonucleotide sequences for TLR9 inhibitory activity in human cells: lack of correlation with TLR9 binding.

International immunology

Ashman RF,Goeken JA,Latz E,Lenert P

14-7061 was used in an ELISA assay to explore the use of oligonucleotide sequences for therapeutic purposes in situations where inflammation is enhanced by TLR9.

Tue Mar 01 00:00:00 EST 2011

Development of murine lupus involves the combined genetic contribution of the SLAM and FcgammaR intervals within the Nba2 autoimmune susceptibility locus.

Journal of immunology (Baltimore, Md. : 1950)

Jørgensen TN,Alfaro J,Enriquez HL,Jiang C,Loo WM,Atencio S,Bupp MR,Mailloux CM,Metzger T,Flannery S,Rozzo SJ,Kotzin BL,Rosemblatt M,Bono MR,Erickson LD

14-7061 was used in an ELISpot assay to identify gene intervals within Nba2 that control the development of autoantibody-producing B cells.

Fri Jan 15 00:00:00 EST 2010

Sialoadhesin-positive macrophages bind regulatory T cells, negatively controlling their expansion and autoimmune disease progression.

Journal of immunology (Baltimore, Md. : 1950)

Wu C,Rauch U,Korpos E,Song J,Loser K,Crocker PR,Sorokin LM

14-7061 was used in Flow cytometry/Cell sorting to propose a new direct cell-cell interaction-based mechanism regulating the expansion of Tregs during autoimmune and inflammatory processes.

Fri May 15 00:00:00 EDT 2009

IL-6 produced by immune complex-activated follicular dendritic cells promotes germinal center reactions, IgG responses and somatic hypermutation.

International immunology

Wu Y,El Shikh ME,El Sayed RM,Best AM,Szakal AK,Tew JG

Published figure using IL-6 monoclonal antibody (Product # 14-7061-81) in Neutralization

Mon Jun 01 00:00:00 EDT 2009

Epicutaneous antigen exposure induces a Th17 response that drives airway inflammation after inhalation challenge.

Proceedings of the National Academy of Sciences of the United States of America

He R,Oyoshi MK,Jin H,Geha RS

14-7061 was used in Immunocytochemistry to suggest that in patients with atopic dermatitis, initial cutaneous exposure to antigens may induce the production of IL-17, driving inflammation in the airways.

Tue Oct 02 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: Il-6; Il6

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UniProt ID: (Mouse) P08505

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Entrez Gene ID: (Mouse) 16193

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Function(s)

Process(es)

receptor binding cytokine activity interleukin-6 receptor binding protein binding growth factor activity

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IL-6 Monoclonal Antibody (MP5-20F3), eBioscience™

IL-6 Antibody (14-7061-81) in IS

Product Details

14-7061-81

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (FN)

Blocking Assay (BLOCK)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

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Concentration

Purification

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Contains

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RRID

Product Specific Information

Target Information

Bioinformatics

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