Invitrogen

IL-6 Monoclonal Antibody (MQ2-13A5), eBioscience™

Product Image Gallery

IL-6 Antibody in Immunocytochemistry (ICC/IF) — Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in Immunocytochemistry

IL-6 Antibody in Flow Cytometry (Flow) — Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in Flow Cytometry

IL-6 Antibody in ELISA (ELISA) — Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in ELISA

IL-6 Antibody in T-Cell Activation (TCA) — Fig. 2 IL-6 is a downstream mediator of DAC-induced morphological alterations in an NOTCH1 dependent and independent manner a ICN1 transient overexpression increased IL-6 release in cell supernatants at 72 h. The Y axis indicates IL-6 in pg/ml normalized for 1 x 10 6 cells from triplicate experiments and technical duplicates with error bars indicating standard error of means. b Bladder tumor cells were treated with DAC every 48 h for up to 5 days. DAC caused IL-6 release in bladder tumor cell lines with a robust increase in HT1376 cells. The Y-axis indicates IL-6 in pg/ml normalized for 1 x 10 6 cells from triplicate experiments and technical duplicates with error bars indicating standard error of means. c-e mRNA levels of double-stranded RNA sensors increased in DAC-treated cells. The Y-axis indicates average fold change over control cells from triplicate experiments and technical duplicates with error bars indicating standard error of means. Student's t-test was used to compare control and treated cells. * p < 0.05, ** p < 0.01. f Bright field images of Giemsa staining showed morphological changes in cells with exogenous IL-6. Cells with exogenous IL-6 appeared enlarged and flattened as compared to control cells. The addition of IL-6 monoclonal antibody reduced the appearance of DAC-induced enlarged cell morphology. _________ represents 10 um. g The addition of a monoclonal antibody against IL-6 with DAC treatment inhibited IL-6 release as compared to DAC treatment alone. T

Product Details

14-7069-81

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	-	View 4 publications 4 publications
ELISA (ELISA)	2-8 µg/mL	View 7 publications 7 publications
Neutralization (Neu)	-	View 2 publications 2 publications
T-Cell Activation (TCA)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Rat / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	MQ2-13A5
Conjugate	Unconjugated Alexa Fluor 700 APC eFluor 450 FITC Functional Grade PE PE-Cyanine7 PerCP-eFluor 710
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_468426

Product Specific Information

Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: The MQ2-13A5 antibody has been reported for use in capture of human IL-6 by ELISA, intracellular staining for flow cytometric analysis, and for neutralizing IL-6 bioactivity.

Applications Tested: The MQ2-13A5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-6 (IL-6) in combination with the biotin MQ2-39C3 (13-7068) antibody for detection and recombinant human IL-6 (14-8069) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-8 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

14-7069 was used in an ELISA assay to describe a simple less virulent, epidemic P. aeruginosa cystic fibrosis clinical isolate LESB65 model allowing non-invasive tracking of bacterial infection, and screening of host-responses to infection and therapies.

Fri Jul 02 00:00:00 EDT 2021

TREM2 Alzheimer's variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages.

Alzheimer's research & therapy

Hall-Roberts H,Agarwal D,Obst J,Smith TB,Monzón-Sandoval J,Di Daniel E,Webber C,James WS,Mead E,Davis JB,Cowley SA

14-7069 was used in an ELISA assay to investigated cell phenotypes of the R47H mutant and TREM2 KO in a model of human microglia, and compared their transcriptional signatures, to determine the mechanism by which R47H TREM2 disrupts function.

Mon Nov 16 00:00:00 EST 2020

CD19⁺CD24^hiCD38^hi B Cells Are Expanded in Juvenile Dermatomyositis and Exhibit a Pro-Inflammatory Phenotype After Activation Through Toll-Like Receptor 7 and Interferon-α.

Frontiers in immunology

Piper CJM,Wilkinson MGL,Deakin CT,Otto GW,Dowle S,Duurland CL,Adams S,Marasco E,Rosser EC,Radziszewska A,Carsetti R,Ioannou Y,Beales PL,Kelberman D,Isenberg DA,Mauri C,Nistala K,Wedderburn LR

14-7069-81 was used in Flow Cytometry to identify that immature transitional B cells were significantly expanded in active JDM, actively dividing, and correlated positively with disease activity.

Wed Nov 20 00:00:00 EST 2019

Decitabine, a DNA-demethylating agent, promotes differentiation via NOTCH1 signaling and alters immune-related pathways in muscle-invasive bladder cancer.

Cell death & disease

Ramakrishnan S,Hu Q,Krishnan N,Wang D,Smit E,Granger V,Rak M,Attwood K,Johnson C,Morrison C,Pili R,Chatta G,Guru K,Gueron G,McNally L,Wang J,Woloszynska-Read A

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in T-Cell Activation

Thu Dec 14 00:00:00 EST 2017

Dengue virus NS1 cytokine-independent vascular leak is dependent on endothelial glycocalyx components.

PLoS pathogens

Glasner DR,Ratnasiri K,Puerta-Guardo H,Espinosa DA,Beatty PR,Harris E

14-7069 was used in an ELISA assay to evaluate the relative contributions of inflammatory mediators and endothelial cell-intrinsic pathways in Dengue virus.

Wed Nov 01 00:00:00 EDT 2017

Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells.

Stem cells (Dayton, Ohio)

Jiang C,Zhang Q,Shanti RM,Shi S,Chang TH,Carrasco L,Alawi F,Le AD

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in Immunofluorescence

Fri Sep 01 00:00:00 EDT 2017

Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease.

Proceedings of the National Academy of Sciences of the United States of America

Zhou Q,Yu X,Demirkaya E,Deuitch N,Stone D,Tsai WL,Kuehn HS,Wang H,Yang D,Park YH,Ombrello AK,Blake M,Romeo T,Remmers EF,Chae JJ,Mullikin JC,Güzel F,Milner JD,Boehm M,Rosenzweig SD,Gadina M,Welch SB,Özen S,Topaloglu R,Abinun M,Kastner DL,Aksentijevich I

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in Flow Cytometry

Tue Sep 06 00:00:00 EDT 2016

Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease.

Proceedings of the National Academy of Sciences of the United States of America

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in Flow Cytometry

Tue Sep 06 00:00:00 EDT 2016

Plasmonically amplified bioassay - Total internal reflection fluorescence vs. epifluorescence geometry.

Talanta

Hageneder S,Bauch M,Dostalek J

14706981 was used to assess IL-6 levels by plasmonic amplification using epifluorescence and total internal reflection fluorescence

Mon Aug 15 00:00:00 EDT 2016

Toll-Like Receptor Activation by Generalized Modules for Membrane Antigens from Lipid A Mutants of Salmonella enterica Serovars Typhimurium and Enteritidis.

Clinical and vaccine immunology : CVI

Rossi O,Caboni M,Negrea A,Necchi F,Alfini R,Micoli F,Saul A,MacLennan CA,Rondini S,Gerke C

14-7069 was used in an ELISA assay to investigate the differences between Salmonella enterica strains in terms of TLR activation by generalised modules for membrane antigens from lipid A mutants.

Fri Apr 01 00:00:00 EDT 2016

Activated human valvular interstitial cells sustain interleukin-17 production to recruit neutrophils in infective endocarditis.

Infection and immunity

Yeh CY,Shun CT,Kuo YM,Jung CJ,Hsieh SC,Chiu YL,Chen JW,Hsu RB,Yang CJ,Chia JS

14-7069 was used in Inhibition experiments to assess the role of activated valvular interstitial cells in valvular damage.

Mon Jun 01 00:00:00 EDT 2015

Monocytes from chronic HBV patients react in vitro to HBsAg and TLR by producing cytokines irrespective of stage of disease.

PloS one

Boltjes A,Groothuismink ZM,van Oord GW,Janssen HL,Woltman AM,Boonstra A

14-7069 was used in Flow cytometry/Cell sorting to investigate the in vitro and in vivo effects of hepatitis B virus surface antigen on monocytes from chronic HBV patients.

Tue Jan 06 00:00:00 EST 2015

TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.

PloS one

Reichwald K,Jørgensen TZ,Tougaard P,Skov S

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in Flow Cytometry

Mon Sep 08 00:00:00 EDT 2014

Modulation of endotoxicity of Shigella generalized modules for membrane antigens (GMMA) by genetic lipid A modifications: relative activation of TLR4 and TLR2 pathways in different mutants.

The Journal of biological chemistry

Rossi O,Pesce I,Giannelli C,Aprea S,Caboni M,Citiulo F,Valentini S,Ferlenghi I,MacLennan CA,D'Oro U,Saul A,Gerke C

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in ELISA

Fri Sep 05 00:00:00 EDT 2014

Induction of cytokines in a macrophage cell line by proteins of Clostridium difficile.

FEMS immunology and medical microbiology

Vohra P,Poxton IR

14-7069 was used in an ELISA assay to assess the induction of cytokines in a macrophage cell line in response to different antigens prepared from five Clostridium difficile strains.

Fri Jun 01 00:00:00 EDT 2012

De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid.

Journal of immunology (Baltimore, Md. : 1950)

Wang J,Huizinga TW,Toes RE

14-7069 was used in Neutralization experiments to investigate methods to convert T cells into Treg cells, showing that retinoic acid can be used for de novo generation of Tregs.

Tue Sep 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

UniProt ID: (Human) P05231

Entrez Gene ID: (Human) 3569

Function(s)