Invitrogen

IL-6 Monoclonal Antibody (MQ2-13A5), eBioscience™

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Published Figures (11)

IL-6 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

IL-6 Antibody (14-7069-81) in ICC/IF

Stem cells (Dayton, Ohio) 2017 - Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in Immunocytochemistry

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IL-6 Antibody in Flow Cytometry (Flow) — 4 FIGURE M2-phenotype macrophages produce interleukin 6 (IL-6) in thyroid cancer. A, Flow cytometry showed that IL-6 was distributed in F4/80 staining-positive tumor-associated macrophages (TAMs). B, Flow cytometry showed that IL-6 was distributed in CD206-positive M2-phenotype macrophages. C, Double immunofluorescence analysis illustrated the colocalization of IL-6 and M2 phenotype within thyroid cancer

IL-6 Antibody in ELISA (ELISA) — Figure 4 Survival and phagocytosis are reduced in PLCgamma2 Ko iPSC macrophages, while LPS-induced secretion of inflammatory cytokines TNFalpha and IL-6 is unaffected. ( A ) Cell death is slightly increased under normal culture conditions after >= 7 days in the PLCgamma2 Ko line compared to the Parent. n = 3 ( B ) In the absence of M-CSF, PLCgamma2 Ko cells show enhanced sensitivity to cell death, as the percentage of dead cells is increasing earlier than in the Parent line. n = 3 ( C ) Representative live-cell images of human iPSC macrophages phagocytosing pHrodo-labelled synaptosomes and quantification of phagocytic uptake indicated as number of spots per cell. PLCgamma2 Ko cells show decreased phagocytosis rate compared to Parent. Cytochalasin D (cytoD, 10 uM) pre-incubation prevents phagocytic uptake in both lines. n = 4. Scale bar 50 mum, inset is a section of the image magnified threefold. ( D) Representative live-cell images of macrophages phagocytosing pHrodo-labelled dead SHSY5Y and quantification of phagocytic uptake indicated as number of spots per cell. PLCgamma2 Ko cells show decreased phagocytosis rate compared to Parent. Cytochalasin D pre-incubation prevents phagocytic uptake in both lines. n = 4. Scale bar 50 mum, inset is a section of the image magnified threefold. ( E) Secretion of TNFalpha and IL-6 after 24 h stimulation with LPS is not affected in the absence of PLCgamma2, n = 4. n.d. - not detectable. Data shown represent mean +- SEM, two-way ANOVA follo

IL-6 Antibody in T-Cell Activation (TCA) — Fig. 2 IL-6 is a downstream mediator of DAC-induced morphological alterations in an NOTCH1 dependent and independent manner a ICN1 transient overexpression increased IL-6 release in cell supernatants at 72 h. The Y axis indicates IL-6 in pg/ml normalized for 1 x 10 6 cells from triplicate experiments and technical duplicates with error bars indicating standard error of means. b Bladder tumor cells were treated with DAC every 48 h for up to 5 days. DAC caused IL-6 release in bladder tumor cell lines with a robust increase in HT1376 cells. The Y-axis indicates IL-6 in pg/ml normalized for 1 x 10 6 cells from triplicate experiments and technical duplicates with error bars indicating standard error of means. c-e mRNA levels of double-stranded RNA sensors increased in DAC-treated cells. The Y-axis indicates average fold change over control cells from triplicate experiments and technical duplicates with error bars indicating standard error of means. Student's t-test was used to compare control and treated cells. * p < 0.05, ** p < 0.01. f Bright field images of Giemsa staining showed morphological changes in cells with exogenous IL-6. Cells with exogenous IL-6 appeared enlarged and flattened as compared to control cells. The addition of IL-6 monoclonal antibody reduced the appearance of DAC-induced enlarged cell morphology. _________ represents 10 um. g The addition of a monoclonal antibody against IL-6 with DAC treatment inhibited IL-6 release as compared to DAC treatment alone. T

Product Details

14-7069-81

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 5 publications 5 publications

ELISA (ELISA)

2-8 µg/mL

View 8 publications 8 publications

Neutralization (Neu)

View 2 publications 2 publications

Inhibition Assays (IA)

View 1 publication 1 publication

T-Cell Activation (TCA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MQ2-13A5

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_468426

Product Specific Information

Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: The MQ2-13A5 antibody has been reported for use in capture of human IL-6 by ELISA, intracellular staining for flow cytometric analysis, and for neutralizing IL-6 bioactivity.

Applications Tested: The MQ2-13A5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-6 (IL-6) in combination with the biotin MQ2-39C3 (13-7068) antibody for detection and recombinant human IL-6 (14-8069) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-8 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Interleukin 6 regulates the expression of programmed cell death ligand 1 in thyroid cancer.

Cancer science

Zhang GQ,Jiao Q,Shen CT,Song HJ,Zhang HZ,Qiu ZL,Luo QY

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

PLCγ2 regulates TREM2 signalling and integrin-mediated adhesion and migration of human iPSC-derived macrophages.

Scientific reports

Obst J,Hall-Roberts HL,Smith TB,Kreuzer M,Magno L,Di Daniel E,Davis JB,Mead E

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in ELISA

Wed Oct 06 00:00:00 EDT 2021

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

14-7069 was used in an ELISA assay to describe a simple less virulent, epidemic P. aeruginosa cystic fibrosis clinical isolate LESB65 model allowing non-invasive tracking of bacterial infection, and screening of host-responses to infection and therapies.

Fri Jul 02 00:00:00 EDT 2021

PTPN2 Regulates Interactions Between Macrophages and Intestinal Epithelial Cells to Promote Intestinal Barrier Function.

Gastroenterology

Spalinger MR,Sayoc-Becerra A,Santos AN,Shawki A,Canale V,Krishnan M,Niechcial A,Obialo N,Scharl M,Li J,Nair MG,McCole DF

14-7069-81 was used in Inhibition Assays to investigate whether PTPN2 controls interactions between IECs and macrophages to maintain intestinal barrier function.

Sun Nov 01 00:00:00 EDT 2020

TREM2 Alzheimer's variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages.

Alzheimer's research & therapy

Hall-Roberts H,Agarwal D,Obst J,Smith TB,Monzón-Sandoval J,Di Daniel E,Webber C,James WS,Mead E,Davis JB,Cowley SA

14-7069 was used in an ELISA assay to investigated cell phenotypes of the R47H mutant and TREM2 KO in a model of human microglia, and compared their transcriptional signatures, to determine the mechanism by which R47H TREM2 disrupts function.

Mon Nov 16 00:00:00 EST 2020

CD19⁺CD24^hiCD38^hi B Cells Are Expanded in Juvenile Dermatomyositis and Exhibit a Pro-Inflammatory Phenotype After Activation Through Toll-Like Receptor 7 and Interferon-α.

Frontiers in immunology

Piper CJM,Wilkinson MGL,Deakin CT,Otto GW,Dowle S,Duurland CL,Adams S,Marasco E,Rosser EC,Radziszewska A,Carsetti R,Ioannou Y,Beales PL,Kelberman D,Isenberg DA,Mauri C,Nistala K,Wedderburn LR

14-7069-81 was used in Flow Cytometry to identify that immature transitional B cells were significantly expanded in active JDM, actively dividing, and correlated positively with disease activity.

Wed Nov 20 00:00:00 EST 2019

Dengue virus NS1 cytokine-independent vascular leak is dependent on endothelial glycocalyx components.

PLoS pathogens

Glasner DR,Ratnasiri K,Puerta-Guardo H,Espinosa DA,Beatty PR,Harris E

14-7069 was used in an ELISA assay to evaluate the relative contributions of inflammatory mediators and endothelial cell-intrinsic pathways in Dengue virus.

Wed Nov 01 00:00:00 EDT 2017

Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells.

Stem cells (Dayton, Ohio)

Jiang C,Zhang Q,Shanti RM,Shi S,Chang TH,Carrasco L,Alawi F,Le AD

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in Immunofluorescence

Fri Sep 01 00:00:00 EDT 2017

Decitabine, a DNA-demethylating agent, promotes differentiation via NOTCH1 signaling and alters immune-related pathways in muscle-invasive bladder cancer.

Cell death & disease

Ramakrishnan S,Hu Q,Krishnan N,Wang D,Smit E,Granger V,Rak M,Attwood K,Johnson C,Morrison C,Pili R,Chatta G,Guru K,Gueron G,McNally L,Wang J,Woloszynska-Read A

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in T-Cell Activation

Thu Dec 14 00:00:00 EST 2017

Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease.

Proceedings of the National Academy of Sciences of the United States of America

Zhou Q,Yu X,Demirkaya E,Deuitch N,Stone D,Tsai WL,Kuehn HS,Wang H,Yang D,Park YH,Ombrello AK,Blake M,Romeo T,Remmers EF,Chae JJ,Mullikin JC,Güzel F,Milner JD,Boehm M,Rosenzweig SD,Gadina M,Welch SB,Özen S,Topaloglu R,Abinun M,Kastner DL,Aksentijevich I

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in ELISA

Tue Sep 06 00:00:00 EDT 2016

Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease.

Proceedings of the National Academy of Sciences of the United States of America

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in ELISA

Tue Sep 06 00:00:00 EDT 2016

Plasmonically amplified bioassay - Total internal reflection fluorescence vs. epifluorescence geometry.

Talanta

Hageneder S,Bauch M,Dostalek J

14706981 was used to assess IL-6 levels by plasmonic amplification using epifluorescence and total internal reflection fluorescence

Mon Aug 15 00:00:00 EDT 2016

Toll-Like Receptor Activation by Generalized Modules for Membrane Antigens from Lipid A Mutants of Salmonella enterica Serovars Typhimurium and Enteritidis.

Clinical and vaccine immunology : CVI

Rossi O,Caboni M,Negrea A,Necchi F,Alfini R,Micoli F,Saul A,MacLennan CA,Rondini S,Gerke C

14-7069 was used in an ELISA assay to investigate the differences between Salmonella enterica strains in terms of TLR activation by generalised modules for membrane antigens from lipid A mutants.

Fri Apr 01 00:00:00 EDT 2016

Activated human valvular interstitial cells sustain interleukin-17 production to recruit neutrophils in infective endocarditis.

Infection and immunity

Yeh CY,Shun CT,Kuo YM,Jung CJ,Hsieh SC,Chiu YL,Chen JW,Hsu RB,Yang CJ,Chia JS

14-7069 was used in Inhibition experiments to assess the role of activated valvular interstitial cells in valvular damage.

Mon Jun 01 00:00:00 EDT 2015

TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.

PloS one

Reichwald K,Jørgensen TZ,Tougaard P,Skov S

Published figure using IL-6 monoclonal antibody (Product # 14-7069-81) in Flow Cytometry

Mon Sep 08 00:00:00 EDT 2014

Modulation of endotoxicity of Shigella generalized modules for membrane antigens (GMMA) by genetic lipid A modifications: relative activation of TLR4 and TLR2 pathways in different mutants.

The Journal of biological chemistry

Rossi O,Pesce I,Giannelli C,Aprea S,Caboni M,Citiulo F,Valentini S,Ferlenghi I,MacLennan CA,D'Oro U,Saul A,Gerke C

14-7069 was used in an ELISA assay to investigate the use of gram-negative bacteria as vaccine candidates.

Fri Sep 05 00:00:00 EDT 2014

Monocytes from chronic HBV patients react in vitro to HBsAg and TLR by producing cytokines irrespective of stage of disease.

PloS one

Boltjes A,Groothuismink ZM,van Oord GW,Janssen HL,Woltman AM,Boonstra A

14-7069 was used in Flow cytometry/Cell sorting to investigate the in vitro and in vivo effects of hepatitis B virus surface antigen on monocytes from chronic HBV patients.

Tue Jan 06 00:00:00 EST 2015

Induction of cytokines in a macrophage cell line by proteins of Clostridium difficile.

FEMS immunology and medical microbiology

Vohra P,Poxton IR

14-7069 was used in an ELISA assay to assess the induction of cytokines in a macrophage cell line in response to different antigens prepared from five Clostridium difficile strains.

Fri Jun 01 00:00:00 EDT 2012

De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid.

Journal of immunology (Baltimore, Md. : 1950)

Wang J,Huizinga TW,Toes RE

14-7069 was used in Neutralization experiments to investigate methods to convert T cells into Treg cells, showing that retinoic acid can be used for de novo generation of Tregs.

Tue Sep 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

UniProt ID: (Human) P05231

Entrez Gene ID: (Human) 3569

Function(s)

Process(es)

cytokine activity interleukin-6 receptor binding protein binding growth factor activity

IL-6 Monoclonal Antibody (MQ2-13A5), eBioscience™

IL-6 Antibody (14-7069-81) in ICC/IF

Product Details

14-7069-81

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Inhibition Assays (IA)

T-Cell Activation (TCA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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