Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Immunocytochemistry (ICC/IF) |
- | View 1 publication 1 publication |
Flow Cytometry (Flow) |
- | View 4 publications 4 publications |
ELISA (ELISA) |
2-8 µg/mL | View 7 publications 7 publications |
Neutralization (Neu) |
- | View 2 publications 2 publications |
T-Cell Activation (TCA) |
- | View 1 publication 1 publication |
Miscellaneous PubMed (Misc) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Human, Mouse |
Host/Isotype |
Rat / IgG1, kappa |
Class |
Monoclonal |
Type |
Antibody |
Clone |
MQ2-13A5 |
Conjugate |
Unconjugated
|
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C |
RRID |
AB_468426 |
Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.
Applications Reported: The MQ2-13A5 antibody has been reported for use in capture of human IL-6 by ELISA, intracellular staining for flow cytometric analysis, and for neutralizing IL-6 bioactivity.
Applications Tested: The MQ2-13A5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-6 (IL-6) in combination with the biotin MQ2-39C3 (13-7068) antibody for detection and recombinant human IL-6 (14-8069) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-8 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6
Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6
UniProt ID: (Human) P05231
Entrez Gene ID: (Human) 3569
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