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Immunofluorescence analysis of IL-8 was performed using 70% confluent log phase U-87 MG cells treated 1 uM thapsigargin for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with IL-8 (3IL8-H10) Mouse Monoclonal Antibody (M801) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification..
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Recombinant human IL-8|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
M801 targets IL-8 in ELISA, and WB applications and shows reactivity with Human samples.
The M801 immunogen is recombinant human IL-8.
M801 detects IL-8 which has a predicted molecular weight of approximately 9 kDa.
The M801 IL8 antibody (clone 3IL8-H10) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M802B (biotinylated conjugate of clone I8-S2). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody M801 (clone 3IL8-H10) and biotinylated antibody M802B (clone I8-S2) have successfully been used in combination with recombinant IL8 protein SIL8 in ELISA applications.
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Transforming growth factor-β impairs glucocorticoid activity in the A549 lung adenocarcinoma cell line.
M801 was used in ELISA to investigate the effect of transforming growth factor-beta on glucocorticoid activity in human A549 cells
|Salem S,Harris T,Mok JS,Li MY,Keenan CR,Schuliga MJ,Stewart AG||British journal of pharmacology (166:2036)||2012|
Ocular fibroblast diversity: implications for inflammation and ocular wound healing.
M801 was used in ELISA to study the significance of ocular fibroblast diversity for inflammation and ocular wound healing
|Xi X,McMillan DH,Lehmann GM,Sime PJ,Libby RT,Huxlin KR,Feldon SE,Phipps RP||Investigative ophthalmology & visual science (52:4859)||2011|
Inhibitors of p38 suppress cytokine production in rheumatoid arthritis synovial membranes: does variable inhibition of interleukin-6 production limit effectiveness in vivo?
M801 was used in ELISA to investigate the role of p38 kinase in rheumatoid arthritis synovial membranes
|Page TH,Brown A,Timms EM,Foxwell BM,Ray KP||Arthritis and rheumatism (62:3221)||2010|
Intermittent haemodiafiltration in refractory congestive heart failure: BNP and balance of inflammatory cytokines.
M801 was used in ELISA to assess the interleukin-8 level in patients with decompensated congestive heart failure
|Libetta C,Sepe V,Zucchi M,Pisacco P,Cosmai L,Meloni F,Campana C,Rampino T,Monti C,Tavazzi L,Dal Canton A||Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (22:2013)||2007|
IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.
M801 was used in ELISA to study the role of mitochondrial pathway in neutrophil survival
|Saffar AS,Alphonse MP,Shan L,Hayglass KT,Simons FE,Gounni AS||Journal of immunology (Baltimore, Md. : 1950) (178:2535)||2007|
Protein microarray platform for the multiplex analysis of biomarkers in human sera.
M801 was used in ELISA to develop a protein microarray platform for the multiplex analysis of biomarkers in human sera
|Urbanowska T,Mangialaio S,Zickler C,Cheevapruk S,Hasler P,Regenass S,Legay F||Journal of immunological methods (316:1)||2006|
|Human||1 ug ml||
Oxidized ATP (oATP) attenuates proinflammatory signaling via P2 receptor-independent mechanisms.
M801 was used in ELISA to study the role of oxidized ATP in proinflammatory signaling
|Beigi RD,Kertesy SB,Aquilina G,Dubyak GR||British journal of pharmacology (140:507)||2003|
Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.
M801 was used in ELISA to investigate the effect of immunosuppressive regimens on cytokine expression in lung transplanted patients
|Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M||Transplantation proceedings (35:1523)||2003|
Development of protein microarray technology to monitor biomarkers of rheumatoid arthritis disease.
M801 was used in ELISA to develop protein microarray technology to monitor biomarkers of rheumatoid arthritis disease
|Urbanowska T,Mangialaio S,Hartmann C,Legay F||Cell biology and toxicology (19:189)||2003|
Regulation of interleukin 8 expression in human malignant melanoma cells.
M801 was used in ELISA to investigate the regulation of interleukin 8 expression in melanoma cells
|Singh RK,Varney ML||Cancer research (58:1532)||1998|
Antineutrophil cytoplasmic antibodies induce monocyte IL-8 release. Role of surface proteinase-3, alpha1-antitrypsin, and Fcgamma receptors.
M801 was used in ELISA to investigate the role of surface proteinase-3, alpha1-antitrypsin, and Fcgamma receptors in monocyte interleukin 8 release
|Ralston DR,Marsh CB,Lowe MP,Wewers MD||The Journal of clinical investigation (100:1416)||1997|
Secretion of proinflammatory cytokines by epithelial cells in response to Chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis.
M801 was used in ELISA to investigate the important roles of epithelial cells in inflammatory response to chlamydia
|Rasmussen SJ,Eckmann L,Quayle AJ,Shen L,Zhang YX,Anderson DJ,Fierer J,Stephens RS,Kagnoff MF||The Journal of clinical investigation (99:77)||1997|
Monocyte Fc gamma receptor cross-linking induces IL-8 production.
M801 was used in ELISA to investigate the influence of monocyte Fc gamma receptor cross-linking on the expression of interleukin 8
|Marsh CB,Gadek JE,Kindt GC,Moore SA,Wewers MD||Journal of immunology (Baltimore, Md. : 1950) (155:3161)||1995|
Rapamycin treatment depresses intragraft expression of KC/MIP-2, granzyme B, and IFN-gamma in rat recipients of cardiac allografts.
M801 was used in immunohistochemistry to investigate the effects of rapamycin on KC/MIP-2, granzyme B, and interferon gamma expression on cardiac allografts
|Wieder KJ,Hancock WW,Schmidbauer G,Corpier CL,Wieder I,Kobzik L,Strom TB,Kupiec-Weglinski JW||Journal of immunology (Baltimore, Md. : 1950) (151:1158)||1993|
alveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; emoctakin; granulocyte chemotactic protein 1; interleukin 8; interleukin-8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; lymphocyte-derived neutrophil-activating factor; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; neutrophil-activating peptide 1; small inducible cytokine subfamily B, member 8; T cell chemotactic factor; T-cell chemotactic factor; tumor necrosis factor-induced gene 1
CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1