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Sandwich ELISA analysis of human IL-8 was performed using a Human IL-8 Colorimetric ELISA kit (Product # EH2IL8) by loading 50 ul per well of Human IL-8 Recombinant Protein (Product # SIL8) in dodecuplicate at 1000, 400, 160, 64, 25.6, and 0 pg/ml across a 3 ug/ml mouse anti-Human IL-8 (Product # M801) pre-coated plate and incubating for 1 hour at room temperature. The plate was washed, and then incubated with 50 ul per well of a biotinylated mouse anti-human IL-8 monoclonal antibody (Product # M802B) in duplicate at 0.25 ug/ml for 1 hour at room temperature. The plate was washed and incubated with 100 ul per well of Streptavidin-HRP (Product # N504) in all test wells at a 1:4,000 dilution for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Recombinant human IL-8|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 7 publications below|
M802B targets IL-8 in ELISA, and WB applications and shows reactivity with Human samples.
The M802B immunogen is recombinant human IL-8.
M802B detects IL-8 which has a predicted molecular weight of approximately 9 kDa.
The M802B IL8 antibody (biotinylated conjugate of clone I8-S2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M801 (clone 3IL8-H10). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody M802B (clone I8-S2) and antibody M801 (clone 3IL8-H10) have successfully been used in combination with recombinant IL8 protein SIL8 in ELISA applications.
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Transforming growth factor-β impairs glucocorticoid activity in the A549 lung adenocarcinoma cell line.
M802B was used in ELISA to investigate the effect of transforming growth factor-beta on glucocorticoid activity in human A549 cells
|Salem S,Harris T,Mok JS,Li MY,Keenan CR,Schuliga MJ,Stewart AG||British journal of pharmacology (166:2036)||2012|
Optical protein sensor for detecting cancer markers in saliva.
M802B was used in ELISA to develop a new sensor for cancer detection in saliva
|Tan W,Sabet L,Li Y,Yu T,Klokkevold PR,Wong DT,Ho CM||Biosensors & bioelectronics (24:266)||2008|
IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.
M802B was used in ELISA to study the role of mitochondrial pathway in neutrophil survival
|Saffar AS,Alphonse MP,Shan L,Hayglass KT,Simons FE,Gounni AS||Journal of immunology (Baltimore, Md. : 1950) (178:2535)||2007|
Protein microarray platform for the multiplex analysis of biomarkers in human sera.
M802B was used in ELISA to develop a protein microarray platform for the multiplex analysis of biomarkers in human sera
|Urbanowska T,Mangialaio S,Zickler C,Cheevapruk S,Hasler P,Regenass S,Legay F||Journal of immunological methods (316:1)||2006|
Oxidized ATP (oATP) attenuates proinflammatory signaling via P2 receptor-independent mechanisms.
M802B was used in ELISA to study the role of oxidized ATP in proinflammatory signaling
|Beigi RD,Kertesy SB,Aquilina G,Dubyak GR||British journal of pharmacology (140:507)||2003|
Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.
M802B was used in ELISA to investigate the effect of immunosuppressive regimens on cytokine expression in lung transplanted patients
|Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M||Transplantation proceedings (35:1523)||2003|
Regulation of interleukin 8 expression in human malignant melanoma cells.
M802B was used in ELISA to investigate the regulation of interleukin 8 expression in melanoma cells
|Singh RK,Varney ML||Cancer research (58:1532)||1998|
alveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; emoctakin; granulocyte chemotactic protein 1; interleukin 8; interleukin-8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; lymphocyte-derived neutrophil-activating factor; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; neutrophil-activating peptide 1; small inducible cytokine subfamily B, member 8; T cell chemotactic factor; T-cell chemotactic factor; tumor necrosis factor-induced gene 1
CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1