|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified recombinant human IL-8.|
|Storage buffer||PBS, pH 7.5, with 5mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 6 publications below|
This product is recommended as a detection antibody in Sandwich ELISA applications.
Interleukin 8 is a pro-inflammatory factor which belongs to the CXC subfamily of chemokinesk, and which are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and is a potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells.
AHC0784 was used in ELISA to report that SFKs regulate the chemokine induction activity of LL-37 in monocytic cells.
|Nijnik A,Pistolic J,Cho P,Filewod NC,Falsafi R,Ramin A,Harder KW,Hancock RE||Journal of leukocyte biology (91:599)||2012|
Rheumatoid factor and its interference with cytokine measurements: problems and solutions.
AHC0784 was used in ELISA to study cytokine levels in patients with rheumatoid arthritis and determine the effect of interference of rheumatoid factor.
|Bartels EM,Falbe Wätjen I,Littrup Andersen E,Danneskiold-Samsøe B,Bliddal H,Ribel-Madsen S||Arthritis (2011:null)||2011|
IL-8 and cathepsin B as melanoma serum biomarkers.
AHC0784 was used in ELISA to study IL8 and Cathepsin B in the prognosis of melanoma mortality.
|Zhang H,Fu T,McGettigan S,Kumar S,Liu S,Speicher D,Schuchter L,Xu X||International journal of molecular sciences (12:1505)||2011|
|Not Applicable||Not Cited||
Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment.
AHC0784 was used in ELISA to optimize innate defense regulator derivatives with enhanced chemokine induction
|Nijnik A,Madera L,Ma S,Waldbrook M,Elliott MR,Easton DM,Mayer ML,Mullaly SC,Kindrachuk J,Jenssen H,Hancock RE||Journal of immunology (Baltimore, Md. : 1950) (184:2539)||2010|
|Not Applicable||Not Cited||
Microbial mannan inhibits bacterial killing by macrophages: a possible pathogenic mechanism for Crohn's disease.
AHC0784 was used in ELISA to evaluate whether Saccharomyces cerevisiae mannan protein inhibits bacterial killing by macrophages as a pathogenic mediator of Crohn's disease
|Mpofu CM,Campbell BJ,Subramanian S,Marshall-Clarke S,Hart CA,Cross A,Roberts CL,McGoldrick A,Edwards SW,Rhodes JM||Gastroenterology (133:1487)||2007|
Igf-I inhibits spontaneous apoptosis in human granulocytes.
AHC0784 was used in ELISA to examine the effects of IGF-I on in vitro apoptosis of human peripheral blood granulocytes.
|Kooijman R,Coppens A,Hooghe-Peters E||Endocrinology (143:1206)||2002|