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Invitrogen
| Applications | Tested Dilution | Publications |
|---|---|---|
Flow Cytometry (Flow) |
0.5 µg/test | View 1 publication 1 publication |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Published species |
Human |
Host/Isotype |
Mouse / IgG1 |
Recommended Isotype Control |
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
Class |
Monoclonal |
Type |
Antibody |
Clone |
CRM17 |
Conjugate |
FITC |
Excitation/Emission Profile |
View spectra
|
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_465375 |
Description: The CRM17 antibody reacts with human interleukin-1 receptor antagonist (IL-1RA). IL-1RA is produced by macrophages, monocytes, neutrophils, synovial and dermal fibroblasts. The 17 kDa soluble form of IL-1RA (sIL-1RA) is produced by hepatocytes. IL-1RA inhibits the release of both forms of IL-1, the secretion of IL-2, and the expression of IL-2 receptors on the cell surface. IL-1RA also blocks the stimulation of prostaglandin E2 synthesis in synovial cells and the proliferation of thymocytes.
Applications Reported: The CRM17 antibody has been reported for use in intracellular staining with flow cytometric analysis.
Applications Tested: The fluorochrome-conjugated CRM17 antibody has been tested by intracellular staining and flow cytometric analysis of LPS-activated, fixed/permeabilized human peripheral blood mononuclear cells. It is offered in 2 formats: - µg size: This can be used at at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested. This can be used at test size: 5 µL (0.25 µg) per test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Protein Aliases: Anakinra; CD121A; D2S1473; ICIL-1RA; IL 1 receptor antagonist; il-1 receptor; il-1r; IL-1RN; IL1 inhibitor; il1 receptor; IL1 receptor antagonist; IL1-RA; IL1R; IL1RN (IL1F3); interleukin-1 receptor; Interleukin-1 receptor antagonist protein; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); MGC10430; P80; type II interleukin-1 receptor antagonist
Gene Aliases: DIRA; ICIL-1RA; IL-1ra; IL-1ra3; IL-1RN; IL1F3; IL1RA; IL1RN; IRAP; MVCD4
UniProt ID: (Human) P18510
Entrez Gene ID: (Human) 3557
Molecular Function:
interleukin superfamily
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