Western blot analysis of IL22 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of GFP-human IL22-transfected 293T cells. Samples were loaded onto a 12% SDS-PAGE gel and probed with an IL22 polyclonal antibody (Product # PA5-34944) at a dilution of 1:5000.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide contains a sequence corresponding to a region within amino acids 1 and 97 of IL22|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34944 targets IL22 in WB applications and shows reactivity with Human and Mouse samples.
The PA5-34944 immunogen is synthetic peptide contains a sequence corresponding to a region within amino acids 1 and 97 of IL22.
IL22 is a cytokine structurally related to IL10 and was originally identified in the mouse as a gene induced by IL9 in T cells and mast cells. IL22 is upregulated in activated T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.