|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Glycosylated, CHO-derived recombinant human GM-CSF|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 9 publications below|
M501 targets Human GM-CSF in ELISA applications and shows reactivity with Human samples.
The M501 immunogen is glycosylated, CHO-derived recombinant human GM-CSF.
M501 detects Human GM-CSF which has a predicted molecular weight of approximately 14 kDa.
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism.
M501 was used in ELISA to study the role of human mast cells in the recruitment of natural killer cells
|Burke SM,Issekutz TB,Mohan K,Lee PW,Shmulevitz M,Marshall JS||Blood (111:5467)||2008|
Serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women.
M501 was used in ELISA to investigate the relationship between serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women
|Tsunoda M,Litonjua AA,Kuniak MP,Weiss ST,Satoh T,Guevarra L,Tollerud DJ||International archives of allergy and immunology (130:158)||2003|
Transgenic overexpression of human IL-17E results in eosinophilia, B-lymphocyte hyperplasia, and altered antibody production.
M501 was used in ELISA to investigate the effect of human interleukin 17E overexpression in transgenic mice
|Kim MR,Manoukian R,Yeh R,Silbiger SM,Danilenko DM,Scully S,Sun J,DeRose ML,Stolina M,Chang D,Van GY,Clarkin K,Nguyen HQ,Yu YB,Jing S,Senaldi G,Elliott G,Medlock ES||Blood (100:2330)||2002|
The Trypanosoma cruzi trans-sialidase is a T cell-independent B cell mitogen and an inducer of non-specific Ig secretion.
M501 was used in ELISA to study the role of Trypanosoma cruzi trans-sialidase in B cell proliferation and Ig secretion
|Gao W,Wortis HH,Pereira MA||International immunology (14:299)||2002|
A mouse thymic stromal cell line producing macrophage-colony stimulating factor and interleukin-6.
M501 was used in ELISA to detect M-CSF and interleukin 6 in a mouse thymic stromal cell line
|Lee CK,Kim JK,Kim K,Han SS||Archives of pharmacal research (23:252)||2000|
The effect of T1 and T2 cytokines on the cytotoxic T cell response to mannan-MUC1.
M501 was used in ELISA to investigate the effect of T1 and T2 cytokines on the cytotoxic T cell response to mannan-MUC1
|Lees CJ,Apostolopoulos V,Acres B,Ong CS,Popovski V,McKenzie IF||Cancer immunology, immunotherapy : CII (48:644)||2000|
Antitumor efficacy of adenocarcinoma cells engineered to produce interleukin 12 (IL-12) or other cytokines compared with exogenous IL-12.
M501 was used in ELISA to investigate the antitumor efficacy of adenocarcinoma cells engineered to produce interleukin 12 or other cytokines
|Cavallo F,Signorelli P,Giovarelli M,Musiani P,Modesti A,Brunda MJ,Colombo MP,Forni G||Journal of the National Cancer Institute (89:1049)||1997|
The IgV domain of human B7-2 (CD86) is sufficient to co-stimulate T lymphocytes and induce cytokine secretion.
M501 was used in ELISA to investigate the role of the IgV domain of human B7-2 in T lymphocyte stimulation and cytokine secretion
|Rennert P,Furlong K,Jellis C,Greenfield E,Freeman GJ,Ueda Y,Levine B,June CH,Gray GS||International immunology (9:805)||1997|
Local and systemic response of mice to interferon-alpha 1-transfected Friend leukemia cells.
M501 was used in ELISA to investigate the effect of injection of interferon-alpha 1-transfected Friend leukemia cells in mice
|Gabriele L,Kaido T,Woodrow D,Moss J,Ferrantini M,Proletti E,Santodonato L,Rozera C,Maury C,Belardelli F||The American journal of pathology (147:445)||1995|