Western blot analysis of ILK using anti-ILK monoclonal antibody (MA5-17228) on (Lane 1) HeLa Cell lysate, (Lane 2) A431 Cell lysate, (Lane 3) MOLT4 Cell lysate, (Lane 4) HT29 Cell lysate, (Lane 5) SK-N-MC Cell lysate, (Lane 6) PC12 Cell lysate and (Lane 7) NIH3T3 Cell lysate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human protein purified from E.coli (His-ILK)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is HeLa cells.
Integrin-linked kinase (ILK) is an adaptor and signaling protein that couples integrins to the actin cytoskeleton and coordinates signal transduction from extracellular matrix and growth factors. By interacting with the focal adhesion proteins PINCH, paxillin, and parvin, ILK regulates integrin-mediated cell adhesion and cytoskeletal dynamics within focal adhesions to regulate cell adhesion, spreading, and migration. ILK is involved in the suppression of apoptosis and promotion of cell survival through the phosphorylation of AKT (Ser473), GSK-3 (Ser9), myosin light chain (Ser18/19), and affixin. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.