|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human INCENP (inner centromere protein)|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Chromosomal passenger proteins are members of a group of proteins that move from centromeres to the spindle midzone during mitosis. INCENP (Inner Centromere Protein) is a chromosomal passenger protein with an essential role in mitosis and meiosis. It is a complex highly basic multidomain protein that is dynamically translocated from metaphase chromosomes to the spindle midzone during or just prior to anaphase. Some INCENP transfers to the equatorial cortex before formation of the cleavage furrow. Ultimately, it is discarded in the midbody at the completion of cytokinesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ser2481-autophosphorylated mTOR colocalizes with chromosomal passenger proteins during mammalian cell cytokinesis.
39-2800 was used in immunocytochemistry to study the colocalization of Ser2481-autophosphorylated mTOR and chromosomal passenger proteins during mammalian cell cytokinesis
|Vazquez-Martin A,Sauri-Nadal T,Menendez OJ,Oliveras-Ferraros C,Cufí S,Corominas-Faja B,López-Bonet E,Menendez JA||Cell cycle (Georgetown, Tex.) (11:4211)||2012|
Enhanced HSP70 lysine methylation promotes proliferation of cancer cells through activation of Aurora kinase B.
39-2800 was used in western blot to report the dimethylation of HSP70 at Lys-561 by SETD1A
|Cho HS,Shimazu T,Toyokawa G,Daigo Y,Maehara Y,Hayami S,Ito A,Masuda K,Ikawa N,Field HI,Tsuchiya E,Ohnuma S,Ponder BA,Yoshida M,Nakamura Y,Hamamoto R||Nature communications (3:null)||2012|
|The active form of the metabolic sensor: AMP-activated protein kinase (AMPK) directly binds the mitotic apparatus and travels from centrosomes to the spindle midzone during mitosis and cytokinesis.||Vazquez-Martin A,Oliveras-Ferraros C,Menendez JA||Cell cycle (Georgetown, Tex.) (8:2385)||2009|