|Immunoprecipitation (IP)||1-10 µg/ml|
|Western Blot (WB)||1-10 µg/ml|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||human placental insulin receptor.|
|Storage buffer||PBS, pH 7|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-10865 detects Insulin Receptor from human samples. Low cross-reactivity with bovine and rabbit samples.
MA1-10865 has been successfully used in Western blot and immunoprecipitation applications.
The MA1-10865 immunogen is human placental insulin receptor.
The insulin receptor is functionally important for negative cooperativity, down-regulation, and beta-subunit tyrosine kinase activity. There are two recognized epitopes of the alpha-subunit. One epitope is close to the insulin-binding site and plays a role in insulin-induced negative cooperativity and down-regulation. The other site (recognized by the monoclonal MA-20 antibody) is at a distance from the insulin-binding site, where it only plays a role in down-regulation. Both epitopes appear to be independent of insulin receptor tyrosine kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD220; HHF5; Insulin receptor; IR
Gene Aliases: CD220; HHF5; INSR
UniProt ID: (Human) P06213
Entrez Gene ID: (Human) 3643
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