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Western blot analysis of Insulin Receptor alpha was performed by loading 25 ug of 293T (lane 1), Hela (lane 2) and PC12 (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Insulin Receptor alpha monoclonal antibody (Product # MA5-13759) at a dilution of 1:200 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~95kDa.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||IM-9 lymphocytes followed by purified insulin receptor|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13756 targets Insulin Receptor alpha in WB, ICC/IF, ELISA and IP applications and shows reactivity with Human samples.
The MA5-13756 immunogen is iM-9 lymphocytes followed by purified insulin receptor.
The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a beta-alpha-alpha-beta configuration. The beta-subunit (95kDa) possesses a single transmembrane domain, whereas the alpha-subunit (135kDa) is completely extracellular.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of death-receptor mediated apoptosis in human adipocytes by the insulin-like growth factor I (IGF-I)/IGF-I receptor autocrine circuit.
MA5-13756 was used in immunoprecipitation to study the role of the IGF-1/IGF-1R autocrine circuit in inhibition of death-receptor mediated apoptosis in human adipocytes
|Fischer-Posovszky P,Tornqvist H,Debatin KM,Wabitsch M||Endocrinology (145:1849)||2004|
An arginine to cysteine(252) mutation in insulin receptors from a patient with severe insulin resistance inhibits receptor internalisation but preserves signalling events.
MA5-13756 was used in immunoprecipitation to study the function of a mutant insulin receptor from a patient with severe insulin resistance
|Hamer I,Foti M,Emkey R,Cordier-Bussat M,Philippe J,De Meyts P,Maeder C,Kahn CR,Carpentier JL||Diabetologia (45:657)||2002|
Increases in free, unbound insulin-like growth factor I enhance insulin responsiveness in human hepatoma G2 cells in culture.
MA5-13756 was used in immunoprecipitation to investigate the effect of a synthetic peptide bp1-01 on the binding of IGF-I to IGFBP-1 and insulin responsiveness in HepG2 cells
|Sakai K,Lowman HB,Clemmons DR||The Journal of biological chemistry (277:13620)||2002|
Glucosamine induces resistance to insulin-like growth factor I (IGF-I) and insulin in Hep G2 cell cultures: biological significance of IGF-I/insulin hybrid receptors.
MA5-13756 was used in blocking or activating experiment to investigate the influence of glucosamine on Hep G2 cell sensitivity to IGF-I and insulin
|Sakai K,Clemmons DR||Endocrinology (144:2388)||2003|
CD220; HHF5; IR; IR alpha
CD220; HHF5; INSR