Immunofluorescent analysis of Insulin Receptor alpha (green) showing staining in the membrane of HepG2 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor alpha monoclonal antibody (Product # MA5-13759) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||IM-9 lymphocytes followed by purified insulin receptor|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13759 targets Insulin Receptor alpha in WB, ICC/IF, ELISA and IP applications and shows reactivity with Human samples.
The MA5-13759 immunogen is iM-9 lymphocytes followed by purified insulin receptor.
The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a beta-alpha-alpha-beta configuration. The beta-subunit (95kDa) possesses a single transmembrane domain, whereas the alpha-subunit (135kDa) is completely extracellular.
IP-MS enrichment of INSR (LFQ intensity): INSR was enriched 505-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and INSR antibody (Part No. MA5-13759). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of death-receptor mediated apoptosis in human adipocytes by the insulin-like growth factor I (IGF-I)/IGF-I receptor autocrine circuit.
MA5-13759 was used in immunoprecipitation to study the role of the IGF-1/IGF-1R autocrine circuit in inhibition of death-receptor mediated apoptosis in human adipocytes
|Fischer-Posovszky P,Tornqvist H,Debatin KM,Wabitsch M||Endocrinology (145:1849)||2004|
An arginine to cysteine(252) mutation in insulin receptors from a patient with severe insulin resistance inhibits receptor internalisation but preserves signalling events.
MA5-13759 was used in immunoprecipitation to study the function of a mutant insulin receptor from a patient with severe insulin resistance
|Hamer I,Foti M,Emkey R,Cordier-Bussat M,Philippe J,De Meyts P,Maeder C,Kahn CR,Carpentier JL||Diabetologia (45:657)||2002|
Increases in free, unbound insulin-like growth factor I enhance insulin responsiveness in human hepatoma G2 cells in culture.
MA5-13759 was used in immunoprecipitation to investigate the effect of a synthetic peptide bp1-01 on the binding of IGF-I to IGFBP-1 and insulin responsiveness in HepG2 cells
|Sakai K,Lowman HB,Clemmons DR||The Journal of biological chemistry (277:13620)||2002|
Glucosamine induces resistance to insulin-like growth factor I (IGF-I) and insulin in Hep G2 cell cultures: biological significance of IGF-I/insulin hybrid receptors.
MA5-13759 was used in blocking or activating experiment to investigate the influence of glucosamine on Hep G2 cell sensitivity to IGF-I and insulin
|Sakai K,Clemmons DR||Endocrinology (144:2388)||2003|