Immunofluorescent analysis of Insulin Receptor alpha (green) showing staining in the cytoplasm and membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor alpha monoclonal antibody (Product # MA5-13767) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Bovine, Human, Sheep, Pig, Rabbit, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||IM-9 lymphocytes followed by IR|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13764 targets Insulin Receptor alpha in ELISA, FACS, ICC/IF and IHC (P) applications and shows reactivity with Bovine, Human, Ovine, Porcine, Rat and Rabbit samples.
The MA5-13764 immunogen is iM-9 lymphocytes followed by IR.
The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a beta-alpha-alpha-beta configuration. The beta-subunit (95kDa) possesses a single transmembrane domain, whereas the alpha-subunit (135kDa) is completely extracellular.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A novel ultra-sensitive enzyme immunoassay for soluble human insulin receptor ectodomain and its measurement in urine from healthy subjects and patients with diabetes mellitus.
MA5-13764 was used in ELISA to evaluate a novel insulin receptor ectodomain detection assay
|Umehara A,Nishioka M,Obata T,Ebina Y,Shiota H,Hashida S||Clinical biochemistry (42:1468)||2009|
Functional insulin receptors on human epithelial ovarian carcinoma cells: implications for IGF-II mitogenic signaling.
MA5-13764 was used in flow cytometry to study the role of functional insulin receptors on human epithelial ovarian carcinoma cells
|Kalli KR,Falowo OI,Bale LK,Zschunke MA,Roche PC,Conover CA||Endocrinology (143:3259)||2002|