Immunofluorescent analysis of Insulin Receptor beta (green) showing staining in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor beta monoclonal antibody (Product # MA5-13778) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A 15-mer synthetic peptide corresponding to aa Tyr-KKNGRILTLPRSNPS from the C-terminal of human insulin receptor beta-subunit|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Affinity Purification (AP)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||2 ug/test|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
MA5-13778 targets Insulin Receptor beta in ELISA, AP, FACS, ICC/IF and IP applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-13778 immunogen is a 15-mer synthetic peptide corresponding to aa Tyr-KKNGRILTLPRSNPS from the C-terminal of human insulin receptor beta-subunit.
The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a beta-alpha-alpha-beta configuration. The beta-subunit (95kDa) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the alpha-subunit (135kDa) is completely extracellular.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Coordinated regulation of insulin signaling by the protein tyrosine phosphatases PTP1B and TCPTP.
MA5-13778 was used in immunoprecipitation to study the coordinated regulation of insulin signaling by the protein tyrosine phosphatases PTP1B and TCPTP
|Galic S,Hauser C,Kahn BB,Haj FG,Neel BG,Tonks NK,Tiganis T||Molecular and cellular biology (25:819)||2005|