Immunofluorescent analysis of Insulin Receptor beta (green) showing staining in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor beta monoclonal antibody (Product # AHR0271) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant fragment including the C-terminal 100 amino acid residues of human insulin receptor.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Staining of formalin-fixed paraffin embedded tissue sections requires boiling the sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A persistent increase in insulin-stimulated glucose uptake by both fast-twitch and slow-twitch skeletal muscles after a single exercise session by old rats.
AHR0271 was used in western blot to measure the effects of exercise on glucose uptake by type I (slow-twitch) muscle from old rats.
|Xiao Y,Sharma N,Arias EB,Castorena CM,Cartee GD||Age (Dordrecht, Netherlands) (35:573)||2013|
Calorie restriction enhances insulin-stimulated glucose uptake and Akt phosphorylation in both fast-twitch and slow-twitch skeletal muscle of 24-month-old rats.
AHR0271 was used in western blot to examine the insulin signaling pathway in isolated epitrochlearis and soleus muscles of old rats.
|Sequea DA,Sharma N,Arias EB,Cartee GD||The journals of gerontology. Series A, Biological sciences and medical sciences (67:1279)||2012|
Preventing the calorie restriction-induced increase in insulin-stimulated Akt2 phosphorylation eliminates calorie restriction's effect on glucose uptake in skeletal muscle.
AHR0271 was used in western blot to examine the effect of Akt2 phosphorylation on on glucose uptake in skeletal muscle
|Sharma N,Arias EB,Sequea DA,Cartee GD||Biochimica et biophysica acta (1822:1735)||2012|
|Not Applicable||Not Cited||
Generation of intracellular domain of insulin receptor tyrosine kinase by gamma-secretase.
AHR0271 was used in western blot to test if the insulin receptor undergoes presenilin/gamma-secretase-dependent processing
|Kasuga K,Kaneko H,Nishizawa M,Onodera O,Ikeuchi T||Biochemical and biophysical research communications (360:90)||2007|
A physiological increase of insulin in the olfactory bulb decreases detection of a learned aversive odor and abolishes food odor-induced sniffing behavior in rats.
AHR0271 was used in immunohistochemistry - frozen section to study the role of insulin in olfactory function.
|Aimé P,Hegoburu C,Jaillard T,Degletagne C,Garcia S,Messaoudi B,Thevenet M,Lorsignol A,Duchamp C,Mouly AM,Julliard AK||PloS one (7:null)||2012|
|Not Applicable||0.75 µg/ml||
In vivo antitumor activity of NVP-AEW541-A novel, potent, and selective inhibitor of the IGF-IR kinase.
AHR0271 was used in ELISA to study the in vivo antitumor activity of a potent and selective IGF-1 receptor kinase inhibitor
|García-Echeverría C,Pearson MA,Marti A,Meyer T,Mestan J,Zimmermann J,Gao J,Brueggen J,Capraro HG,Cozens R,Evans DB,Fabbro D,Furet P,Porta DG,Liebetanz J,Martiny-Baron G,Ruetz S,Hofmann F||Cancer cell (5:231)||2004|