|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant human IL-10|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1:10 -1:50|
|Functional Assay (FN)||Assay Dependent|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01EU/ug.
Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active tran
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Application of six-color flow cytometry for the assessment of dendritic cell responses in whole blood assays.
MA5-16749 was used in flow cytometry to describe a 6-color assay to analyze peripheral blood dendritic cell responses upon whole blood stimulation.
|Della Bella S,Giannelli S,Taddeo A,Presicce P,Villa ML||Journal of immunological methods (339:153)||2008|
A soluble factor produced by lamina propria mononuclear cells is required for TNF-alpha enhancement of IFN-gamma production by T cells.
MA5-16749 was used in ELISA to elucidate the mechanism of TNF-alpha-augmented mucosal T cell IFN-gamma production.
|Prehn JL,Landers CJ,Targan SR||Journal of immunology (Baltimore, Md. : 1950) (163:4277)||1999|