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Immunofluorescence analysis of IQGAP1 Monoclonal Antibody was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with IQGAP1 (AF1) Mouse Monoclonal Antibody (338900) at 2ug/ml in 0.1% BSA, incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Primate|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||A recombinant protein derived from the C-terminal fragment of human IQGAP1 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
DISP2 is the second of three known homologs of the D. melanogaster protein Dispatched. It is a multi-transmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored Hedgehog from producing cells. Hedgehog is a major player in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis and the DISP proteins have been implicated in these pathways. Recently, it has been shown that DISP2 is translationally regulated by the microRNA miR-214 in zebrafish. Expression of this miRNA decreased DISP2 promoter activity in vitro and its overexpression in zebrafish resulted in a phenotype identical to that observed by DISP2 mutants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Patient Mutation Directed shRNA Screen Uncovers Novel Bladder Tumor Growth Suppressors.
33-8900 was used in western blot to develop and use a high-throughput, in vivo strategy to determine which alterations are loss of function in tumor growth suppressors using bladder cancer samples.
|Hensel J,Duex JE,Owens C,Dancik GM,Edwards MG,Frierson HF,Theodorescu D||Molecular cancer research : MCR (13:1306)||2015|
|Mouse||1:25||IQGAP1 scaffold-kinase interaction blockade selectively targets RAS-MAP kinase-driven tumors.||Jameson KL,Mazur PK,Zehnder AM,Zhang J,Zarnegar B,Sage J,Khavari PA||Nature medicine (19:626)||2013|
Cdc42-Rac1 effector protein; DISP2; Dispatched homolog 2; DISPB; ras GTPase-activating-like protein IQGAP1; RasGAP-like with IQ motifs
AA682088; D7Ertd237e; D7Ertd257e; HUMORFA01; IQGAP1; KIAA0051; mKIAA0051; p195; SAR1