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Western blot analysis of IR beta in whole cell extracts of HeLa cells treated with 100ng/mL insulin for 15 min (lane1); no treatment (lane 2); or treated (150ng/mL IGF1 after serum starvation (lane 3) using an IR beta recombinant rabbit oligoclonal antibody (Product # 710076) at a dilution of 2.5ug/ml.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 1283–1382 of human IR beta subunit|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Insulin receptor (IR), belongs to the growth factor receptor tyrosine kinase superfamily which regulates multiple signaling pathways. It is a heterotetrameric membrane glycoprotein consisting of alpha and beta subunits encoded by the INSR gene in humans. IR and IGF-1 receptors share major structural and functional similarity. The earliest cellular response to insulin stimulation is autophosphorylation of tyrosine in IR. In humans, the IR gene is located on chromosome 19.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD220; CD221; HHF5; IGF-I receptor; INSR; insulin receptor; insulin-like growth factor I receptor; IR; soluble IGF1R variant 1; soluble IGF1R variant 2
CD220; HHF5; INSR