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Abnova

IRAK1 Monoclonal Antibody (3F7)

View all (35) IRAK1 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

H00003654-M01

Applications Tested Dilution Publications

Western Blot (WB)

 1-5 µg/mL -

Immunohistochemistry (Paraffin) (IHC (P))

 3 µg/mL -

ELISA (ELISA)

 0.1 ng/mL -

Immunoprecipitation (IP)

 1-5 µg/mL -
Product Specifications

Species Reactivity

 Human

Host/Isotype

 Mouse / IgG2a, kappa

Class

 Monoclonal

Type

 Antibody

Clone

 3F7

Immunogen

IRAK1 (AAH54000, 530 a.a. approximately 693 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Conjugate

 Unconjugated

Form

 Liquid

Concentration

 See Label

Purification

 Affinity chromatography

Storage buffer

 PBS, pH 7.4

Contains

 no preservative

Storage conditions

 -20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Sequence of this protein is as follows: SSTGRAHSGA APWQPLAAPS GASAQAAEQL QRGPNQPVES DESLGGLSAA LRSWHLTPSC PLDPAPLREA GCPQGDTAGE SSWGSGPGSR PTAVEGLALG SSASSSSEPP QIIINPARQK MVQKLALYED GALDSLQLLS SSSLPGLGLE QDRQGPEESD EFQS

Target Information

Interleukin-1 receptor-associated kinase 1 (IRAK1) is a serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. IRAK is associated with Toll-like receptor (TLR) and IL-1R signaling pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Interleukin-1 receptor-associated kinase 1; IRAK-1; Pelle homolog

Gene Aliases: IRAK; IRAK1; pelle

UniProt ID: (Human) P51617

Entrez Gene ID: (Human) 3654

Function(s)
activation of MAPK activity regulation of cytokine-mediated signaling pathway toll-like receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway protein phosphorylation signal transduction transmembrane receptor protein serine/threonine kinase signaling pathway activation of NF-kappaB-inducing kinase activity JNK cascade aging lipopolysaccharide-mediated signaling pathway negative regulation of NF-kappaB transcription factor activity positive regulation of type I interferon production response to lipopolysaccharide toll-like receptor 2 signaling pathway toll-like receptor 4 signaling pathway toll-like receptor 9 signaling pathway cellular response to heat negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAP kinase activity innate immune response positive regulation of transcription, DNA-templated protein autophosphorylation positive regulation of smooth muscle cell proliferation positive regulation of NF-kappaB transcription factor activity protein oligomerization nucleotide-binding oligomerization domain containing signaling pathway interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to hypoxia
Process(es)
protein kinase activity protein serine/threonine kinase activity NF-kappaB-inducing kinase activity protein binding ATP binding kinase activity heat shock protein binding protein homodimerization activity protein heterodimerization activity
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