Invitrogen

IRAK2 Recombinant Rabbit Monoclonal Antibody (JG36-23)

View all (28) IRAK2 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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IRAK2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of IRAK2 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with IRAK2 monoclonal antibody (Product # MA5-34746), followed by DAPI (blue).

IRAK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of IRAK2 in paraffin-embedded human prostate cancer tissue. Samples were incubated with IRAK2 monoclonal antibody (Product # MA5-34746), and followed by hematoxylin.

IRAK2 Antibody in Western Blot (WB) — Western blot analysis of IRAK2 in Positive control: Line 1: PC-3M Line 2: A549 Line 3: HepG2. Samples were incubated with IRAK2 monoclonal antibody (Product # MA5-34746), at a dilution of 1:500.

IRAK2 Antibody in Flow Cytometry (Flow) — Flow cytometry of IRAK2 in Jurkat cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with IRAK2 monoclonal antibody (Product # MA5-34746) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

Product Details

MA5-34746

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-
Immunoprecipitation (IP)	1:10-1:50	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JG36-23
Immunogen	Recombinant protein within Human IRAK2 aa 30-270 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles, store in dark
RRID	AB_2848654

Product Specific Information

Positive Control: PC-3M, A549, HepG2, Jurkat, human tonsil tissue, human prostate cancer tissue, human heart tissue.

Target Information

IRAK2 (interleukin-1 receptor-associated kinase-like 2) binds to the IL-1 type I receptor following IL-1 engagment and triggers intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappa-B, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappa-B activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kappa-B activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory responses to cytokine IL-1 and LPS. IRAK2 is expressed in a variety of human tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: interleukin-1 receptor associated kinase-2; interleukin-1 receptor-associated kinase 2; Interleukin-1 receptor-associated kinase-like 2; IRAK-2; MGC150550

Gene Aliases: IRAK-2; IRAK2

UniProt ID: (Human) O43187

Entrez Gene ID: (Human) 3656

Function(s)

activation of MAPK activity regulation of cytokine-mediated signaling pathway toll-like receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway protein phosphorylation inflammatory response I-kappaB kinase/NF-kappaB signaling JNK cascade lipopolysaccharide-mediated signaling pathway negative regulation of NF-kappaB transcription factor activity toll-like receptor 9 signaling pathway positive regulation of NF-kappaB transcription factor activity nucleotide-binding oligomerization domain containing signaling pathway interleukin-1-mediated signaling pathway response to interleukin-1

Process(es)

protein serine/threonine kinase activity protein binding ATP binding protein homodimerization activity protein heterodimerization activity

IRAK2 Recombinant Rabbit Monoclonal Antibody (JG36-23)

Product Details

MA5-34746

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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