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Invitrogen
IRF1 Recombinant Rabbit Monoclonal Antibody (SR44-08)
FIGURE: 1 / 7
IRF1 Antibody (MA5-31996) in WB
Western blot was performed using Anti-IRF1 Recombinant Rabbit Monoclonal Antibody (SR44-08) (Product # MA5-31996) and a band at ~45 kDa corresponding to IRF1 was induced upon IFN gamma treatment in HeLa and A549. An uncharacterized band (*) at ~35 kDa was observed across the samples tested. Modified whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with IFN gamma (100ng/ml, 4hr) (Lane 2) and A549 (Lane 3), A549 treated with IFN gamma (100ng/ml, 4hr) and Raji (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tri... View More
Product Details
MA5-31996
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SR44-08
Immunogen
Recombinant protein within Human IRF1 aa 86-325
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2809290
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppression. Diseases associated with IRF1 include gastric cancer, somatic and lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Interferon regulatory factor 1; interferon regulatory factor 1 isoform +I9; interferon regulatory factor 1 isoform d78; interferon regulatory factor 1 isoform delta4; interferon regulatory factor 1 isoform delta7; IRF-1
Gene Aliases: AU020929; IRF-1; IRF1; MAR
UniProt ID: (Human) P10914, (Mouse) P15314, (Rat) P23570
Entrez Gene ID: (Human) 3659, (Mouse) 16362, (Rat) 24508
Molecular Function:
winged helix/forkhead transcription factor
regulation of adaptive immune response
transcription from RNA polymerase II promoter
apoptotic process
cell cycle arrest
blood coagulation
negative regulation of cell proliferation
positive regulation of type I interferon production
positive regulation of interferon-beta production
regulation of MyD88-dependent toll-like receptor signaling pathway
cellular response to interferon-beta
CD8-positive, alpha-beta T cell differentiation
positive regulation of interleukin-12 biosynthetic process
regulation of innate immune response
negative regulation of regulatory T cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
regulation of cell cycle
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
cellular response to mechanical stimulus
regulation of CD8-positive, alpha-beta T cell proliferation
immune system process
transcription, DNA-templated
regulation of transcription, DNA-templated
I-kappaB kinase/NF-kappaB signaling
regulation of gene expression
positive regulation of cell death
positive regulation of natural killer cell differentiation
negative regulation of tyrosine phosphorylation of Stat1 protein
innate immune response
positive regulation of T-helper 1 cell differentiation
negative regulation of T-helper 2 cell differentiation
response to growth hormone
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to peptide hormone stimulus
female pregnancy
response to heat
regulation of cell proliferation
cellular response to retinoic acid
cellular response to dsRNA
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
protein binding
regulatory region DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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