Invitrogen

IRF4 Monoclonal Antibody (MUM1p)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 References

View all (35) IRF4 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

IRF4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical (Paraffin) analysis of human tonsil tissue using (Product # MA1-25525) IRF4/MUM1 antibody [MUM1p].

IRF4 Antibody in Western Blot (WB) — Western blot was performed using Anti-IRF4 Monoclonal Antibody (MUM1p) (Product # MA1-25525) and a 51kDa band corresponding to IRF4 was observed in IM9 and not in HEK-293, MCF7 and THP-1 which are reported to be negative. Whole cell extracts (30 µg lysate) of IM9 (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3) and THP-1 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

IRF4 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of IRF4 was observed in IM9 in comparison to HEK-293, MCF7 and THP-1 using Anti-IRF4 Monoclonal antibody (MUM1p) (Product # MA1-25525) in Western Blot. {RE}

Product Details

MA1-25525

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	-
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:25	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	MUM1p
Immunogen	Recombinant MUM1 protein.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	tissue culture supernatant
Contains	0.09% sodium azide
Storage conditions	4° C, do not freeze
RRID	AB_794981

Product Specific Information

Recommended positive controls: Tonsil.

Target Information

The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Significance of stromal-1 and stromal-2 signatures and biologic prognostic model in diffuse large B-cell lymphoma.

Cancer biology & medicine

Abdou AG,Asaad N,Kandil M,Shabaan M,Shams A

MA1-25525 was used in Immunohistochemistry to assess the significance of stromal-1 signature using SPARC-1 and stromal-2 signature.

Mon May 01 00:00:00 EDT 2017

Composite lymphoma in the anterior mediastinum: a case report and review of the literature.

Diagnostic pathology

Yu G,Kong L,Qu G,Zhang Q,Wang W,Jiang L

MA1-25525 was used in immunohistochemistry to present a case report of an unusual composite lymphoma in the mediastinum

Wed Jul 06 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: Interferon regulatory factor 4; IRF-4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; NF-EM5

Gene Aliases: IRF4; LSIRF; MUM1; NF-EM5; SHEP8

UniProt ID: (Human) Q15306

Entrez Gene ID: (Human) 3662

Molecular Function: winged helix/forkhead transcription factor

Function(s)

transcription from RNA polymerase II promoter peptidyl-lysine methylation negative regulation of toll-like receptor signaling pathway T cell activation defense response to protozoan myeloid dendritic cell differentiation positive regulation of DNA binding histone H3 acetylation histone H4 acetylation positive regulation of interleukin-10 biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-13 biosynthetic process positive regulation of interleukin-4 biosynthetic process regulation of T-helper cell differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter interferon-gamma-mediated signaling pathway type I interferon signaling pathway T-helper 17 cell lineage commitment

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein-lysine N-methyltransferase activity sequence-specific DNA binding

IRF4 Monoclonal Antibody (MUM1p)

Product Details

MA1-25525

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!