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FIGURE: 1 / 2
IRF5 Antibody (MA5-35069) in WB
Western blot analysis of IRF5 in extracts of various cell lines. Samples were incubated with IRF5 Monoclonal antibody (Product # MA5-35069) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5 min.
Product Details
MA5-35069
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0525
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 6-151 of human IRF5 (Q13568)
Conjugate
Form
Liquid
Concentration
1.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848974
Product Specific Information
Immunogen sequence: PVAPTPPRRV RLKPWLVAQV NSCQYPGLQW VNGEKKLFCI PWRHATRHGP SQDGDNTIFK AWAKETGKYT EGVDEADPAK WKANLRCALN KSRDFRLIYD GPRDMPPQPY KIYEVCSNGP APTDSQPPED YSFGAGEEEE EEEELQ
Target Information
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Interferon regulatory factor 5; interferon regulatory factor 5 bone marrow; IRF-5; OTTHUMP00000212907
Gene Aliases: AW491843; IRF5; mirf5; SLEB10
UniProt ID: (Human) Q13568, (Mouse) P56477
Entrez Gene ID: (Human) 3663, (Mouse) 27056
transcription, DNA-templated
response to peptidoglycan
response to muramyl dipeptide
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-12 production
positive regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
immune system process
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cytokine-mediated signaling pathway
innate immune response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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