|Tested species reactivity||Mouse|
|Published species reactivity||Non-human primate, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein derived from amino acids 207-452 of the murine IRF7 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Gel Shift (GS)||0.2-1.0 ug per 5 ul|
|Immunofluorescence (IF)||10 ug/ml|
|Immunoprecipitation (IP)||3.0-5.0 ug per 1x10^7 cells extract|
|Western Blot (WB)||0.5-2.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Comparison of tamoxifen and letrozole response in mammary preneoplasia of ER and aromatase overexpressing mice defines an immune-associated gene signature linked to tamoxifen resistance.
51-3300 was used in immunohistochemistry to examine the response and resistance to endocrine therapy in mice.
|Dabydeen SA,Kang K,Díaz-Cruz ES,Alamri A,Axelrod ML,Bouker KB,Al-Kharboosh R,Clarke R,Hennighausen L,Furth PA||Carcinogenesis (36:122)||2015|
Negative regulation of type I IFN expression by OASL1 permits chronic viral infection and CD8¿ T-cell exhaustion.
51-3300 was used in western blot to test if 2'-5' oligoadenylate synthetase-like contributes to the virus-specific T-cell response and viral defense against chronic lymphocytic choriomeningitis virus.
|Lee MS,Park CH,Jeong YH,Kim YJ,Ha SJ||PLoS pathogens (9:null)||2013|
|Mouse||Not Cited||Tissue-specific positive feedback requirements for production of type I interferon following virus infection.||Prakash A,Smith E,Lee CK,Levy DE||The Journal of biological chemistry (280:18651)||2005|
|Acetylation of interferon regulatory factor-7 by p300/CREB-binding protein (CBP)-associated factor (PCAF) impairs its DNA binding.||Caillaud A,Prakash A,Smith E,Masumi A,Hovanessian AG,Levy DE,Marié I||The Journal of biological chemistry (277:49417)||2002|