Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 12
IRF7 Antibody (MA5-41165) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of IRF7 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with IRF7 Recombinant Monoclonal Antibody (Product # MA5-41165) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
Product Details
MA5-41165
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SC0617
Immunogen
Synthetic peptide within human IRF7 aa 200-240
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2898919
Target Information
Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Interferon regulatory factor 7; interferon regulatory factor-7H; IRF-7
Gene Aliases: IMD39; IRF-7H; IRF7; IRF7A; IRF7B; IRF7C; IRF7H
UniProt ID: (Human) Q92985, (Mouse) P70434
Entrez Gene ID: (Human) 3665, (Mouse) 54123, (Rat) 293624
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
protein binding
regulatory region DNA binding
transcription factor activity, sequence-specific DNA binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
regulation of adaptive immune response
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
response to virus
immunoglobulin mediated immune response
establishment of viral latency
regulation of type I interferon production
positive regulation of type I interferon production
interferon-alpha production
interferon-beta production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
regulation of MyD88-dependent toll-like receptor signaling pathway
regulation of MyD88-independent toll-like receptor signaling pathway
TRIF-dependent toll-like receptor signaling pathway
MDA-5 signaling pathway
innate immune response
type I interferon biosynthetic process
regulation of monocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of immune response
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
positive regulation of type I interferon-mediated signaling pathway
negative regulation of macrophage apoptotic process
immune system process
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
regulation of innate immune response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support