Invitrogen

IRF8 Monoclonal Antibody (GW4CML3), eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 Published Figures

1 Reference

View all (28) IRF8 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

IRF8 Antibody in Western Blot (WB) — Western blot was performed using Anti-IRF8 Monoclonal Antibody (GW4CML3), eBioscience™(Product # 14-7888-82) and a 50kDa band corresponding to IRF8 was observed across cell lines and tissue extract tested except K-562, T98G and Mouse Skeletal Muscle which are reported to be negative. Nuclear enriched extracts (30 µg lysate) of THP-1 (Lane 1), Raji (Lane 2), Ramos (Lane 3), K-562 (Lane 4), T98G (Lane 5) and tissue extracts of Mouse Skeletal Muscle (Lane 6) and Mouse Spleen (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2.5 ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

IRF8 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues owing to their inherent genetic constitution. Relative expression of IRF8 was observed in THP-1, Raji, Ramos and Mouse Spleen in comparison to K-562, T98G and Mouse Skeletal Muscle using Anti-IRF8 Monoclonal Antibody (GW4CML3), eBioscience™ (Product # 14-7888-82) in Western Blot. {RE}

Product Details

14-7888-82

Applications	Tested Dilution	Publications
Western Blot (WB)	1-5 µg/mL	View 1 publication 1 publication
Flow Cytometry (Flow)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	GW4CML3
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_2572907

Product Specific Information

Description: The GW4CML3 monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP). IRF8 is a 50 kDa transcription factor that plays a critical role in the development of dendritic cells (DC). Specifically, IRF8 is essential in the development and differentiation of plasmacytoid DC (pDC) and CD8a+ DC. IRF8-/- mice are deficient in both pDC and CD8a+ DC populations. CD8a- DC are present in normal numbers in IRF8-/- mice but fail to mature upon Toll-like receptor signaling and are functionally impaired in response to in vivo microbial stimulation. IRF8 deficiency does not affect the frequency or viability of DC precursors and as a result retroviral IRF8 transduction restores pDC development from DC progenitors in IRF8-/- mice.

Applications Reported: This GW4CML3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated GW4CML3 is recommended for use in intracellular flow cytometry).

Applications Tested: This GW4CML3 antibody has been tested by western blot on lysates from stimulated normal human peripheral blood cells. This can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion.

Cell

Gangoso E,Southgate B,Bradley L,Rus S,Galvez-Cancino F,McGivern N,Güç E,Kapourani CA,Byron A,Ferguson KM,Alfazema N,Morrison G,Grant V,Blin C,Sou I,Marques-Torrejon MA,Conde L,Parrinello S,Herrero J,Beck S,Brandner S,Brennan PM,Bertone P,Pollard JW,Quezada SA,Sproul D,Frame MC,Serrels A,Pollard SM

14-7888-82 was used in Western Blot to conclude that epigenetic immunoediting may drive an acquired immune evasion program in the most aggressive mesenchymal GBM subtype by reshaping the tumor immune microenvironment.

Thu Apr 29 00:00:00 EDT 2021

Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion.

Cell

Thu Apr 29 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: H-ICSBP; ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF-8

Gene Aliases: AI893568; H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; IRF8; Myls

UniProt ID: (Human) Q02556, (Mouse) P23611

Entrez Gene ID: (Human) 3394, (Mouse) 15900

Molecular Function: winged helix/forkhead transcription factor