Invitrogen

IRF8 Monoclonal Antibody (V3GYWCH), Alexa Fluor™ 488, eBioscience™

Product Image Gallery

IRF8 Antibody in Flow Cytometry (Flow) — Surface staining of normal human peripheral blood cells with Anti-Human CD123 Monoclonal Antibody, eFluor 450 (Product # 48-1239-42), followed by intracellular staining using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol with 0.125 µg of Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (left) or 0.125 µg of IRF8 Monoclonal Antibody, Alexa Fluor 488 (right). Cells in the lymphocyte and monocyte gate were used for analysis.

Product Details

53-9852-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.25 µg/test	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Not Applicable
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	V3GYWCH
Conjugate	Alexa Fluor™ 488 APC eFluor 450 PE PE-Cyanine7 PerCP-eFluor 710
Excitation/Emission Max	499/520 nm View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2784763

Product Specific Information

Description: The V3GYWCH monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP). IRF8 is a 50 kDa transcription factor that plays a critical role in the development of dendritic cells (DC). Specifically, IRF8 is essential in the development and differentiation of plasmacytoid DC (pDC) and CD8a+ DC. IRF8-/- mice are deficient in both pDC and CD8a+ DC populations. CD8a- DC are present in normal numbers in IRF8-/- mice but fail to mature upon Toll-like receptor signaling and are functionally impaired in response to in vivo microbial stimulation. IRF8 deficiency does not affect the frequency or viability of DC precursors and as a result retroviral IRF8 transduction restores pDC development from DC progenitors in IRF8-/- mice.

Applications Reported: This V3GYWCH antibody has been reported for use in flow cytometric analysis.

Applications Tested: This V3GYWCH antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Target Information

IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CD103⁺ cDC1 and endogenous CD8⁺ T cells are necessary for improved CD40L-overexpressing CAR T cell antitumor function.

Nature communications

Kuhn NF,Lopez AV,Li X,Cai W,Daniyan AF,Brentjens RJ

Published figure using IRF8 monoclonal antibody (Product # 53-9852-82) in Flow Cytometry

Wed Dec 02 00:00:00 EST 2020

Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population.

Cell reports

Leylek R,Alcántara-Hernández M,Lanzar Z,Lüdtke A,Perez OA,Reizis B,Idoyaga J

Published figure using IRF8 monoclonal antibody (Product # 53-9852-82) in Flow Cytometry

Tue Dec 10 00:00:00 EST 2019

Bioinformatics

Protein Aliases: H-ICSBP; ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF-8

Gene Aliases: AI893568; H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; IRF8; Myls

UniProt ID: (Human) Q02556, (Mouse) P23611

Entrez Gene ID: (Human) 3394, (Mouse) 15900

Molecular Function: winged helix/forkhead transcription factor