|Gel Shift (GS)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:500|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an internal portion of the murine IRP2 protein sequence (between residues 100-200). UniProt Q811J3|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody has 94% sequence homology with human, primate and rat.
Suggested positive control: 3T3 whole cell lysate.
Iron regulatory protein 2 (IRP2) is a central post-transcriptional regulator of cellular and systemic iron metabolism. It is a cytosolic RNA-binding protein that binds to stem-loop structures. IRP2 is regulated by iron-mediated proteolysis. Hypoxia is important for normal tissue physiology as well as being a component of several pathophysiological conditions, including heart and cerebrovascular diseases and tumor growth. Because iron and oxygen are closely related, IRP2 also plays a role during hypoxia (low oxygen).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IRE-BP 2; Iron regulatory protein 2; Iron-responsive element binding protein 2; Iron-responsive element-binding protein 2; IRP2
Gene Aliases: D9Ertd85e; Ireb2; Irp2
UniProt ID: (Mouse) Q811J3
Entrez Gene ID: (Mouse) 64602
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