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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to aa 1223-1242 of human IRS-1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is colon carcinoma.
The insulin receptor substrate-1 (IRS-1), a 170-185kDa protein major substrate of the insulin receptor is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor (IGF-I) and interleukin 4. IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.