|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to aa 1223-1242 of human IRS-1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is colon carcinoma.
The insulin receptor substrate-1 (IRS-1), a 170-185kDa protein major substrate of the insulin receptor is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor (IGF-I) and interleukin 4. IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HIRS-1; Insulin receptor substrate 1; IRS-1
Gene Aliases: HIRS-1; IRS1
UniProt ID: (Human) P35568
Entrez Gene ID: (Human) 3667
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