Immunofluorescence analysis of IFIT2 was performed using 70% confluent log phase HT-1080 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with IFIT2 Rabbit Polyclonal Antibody (PA3845) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3 - 5 µg/10^6 cells|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
PA3-845 detects ISG54 (p54) from human and mouse samples.
PA3-845 has been successfully used in ICC/IF and in Western blot applications to detect a 39-58 kDa band. This antibody may produce multiple background bands, and it is recommended to run samples slowly on an 8% gel to achieve proper separation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Type I IFNs Act upon Hematopoietic Progenitors To Protect and Maintain Hematopoiesis during Pneumocystis Lung Infection in Mice.
PA3-845 was used in immunohistochemistry - frozen section to study pneumocystis lung infection in mice ahd type 1 IFNs acting upon hematopoietic progenitors to maintain and protect hematopoiesis
|Prigge JR,Hoyt TR,Dobrinen E,Capecchi MR,Schmidt EE,Meissner N||Journal of immunology (Baltimore, Md. : 1950) (195:5347)||2015|