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Immunofluorescent analysis of p54 (green) showing staining in the cytoplasm of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a p54 polyclonal antibody (Product # PA3-845) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
PA3-845 detects ISG54 (p54) from human and mouse samples.
PA3-845 has been successfully used in ICC/IF and in Western blot applications to detect a 39-58 kDa band. This antibody may produce multiple background bands, and it is recommended to run samples slowly on an 8% gel to achieve proper separation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Type I IFNs Act upon Hematopoietic Progenitors To Protect and Maintain Hematopoiesis during Pneumocystis Lung Infection in Mice.
PA3-845 was used in immunohistochemistry - frozen section to study pneumocystis lung infection in mice ahd type 1 IFNs acting upon hematopoietic progenitors to maintain and protect hematopoiesis
|Prigge JR,Hoyt TR,Dobrinen E,Capecchi MR,Schmidt EE,Meissner N||Journal of immunology (Baltimore, Md. : 1950) (195:5347)||2015|
GARG-39; Glucocorticoid-attenuated response gene 39 protein; IFI-54K; IFIT-2; IFIT2; Interferon, alpha-inducible protein (MW 54kD); Interferon-induced 54 kDa protein; interferon-induced protein 54; Interferon-induced protein with tetratricopeptide repeats 2; ISG-54 K
AV302338; CIG-42; cig42; G10P2; GARG-39; Garg39; IFI-54; IFI-54K; IFI54; IFIT-2; IFIT2; ISG-54 K; ISG-54K; ISG54; P54