Immunofluorescence analysis of IFIT1 was performed using 70% confluent log phase HT-1080 cells treated with Interferon-beta (1000 U/ml) for 12 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with IFIT1 Rabbit Polyclonal Antibody (PA3848) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the untreated cells that do not have IFIT1 expression. Panel f represents the no primary control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-848 detects ISG56 (p56) from human and mouse samples.
PA3-848 has been successfully used in Western blot and ICC/IF applications to detect a 39-58 kDa band.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Characterization of a Novel Human-Specific STING Agonist that Elicits Antiviral Activity Against Emerging Alphaviruses.
PA3-848 was used in western blot to identify small molecules that activate the type I interferon response via IFN regulatory factor 3
|Sali TM,Pryke KM,Abraham J,Liu A,Archer I,Broeckel R,Staverosky JA,Smith JL,Al-Shammari A,Amsler L,Sheridan K,Nilsen A,Streblow DN,DeFilippis VR||PLoS pathogens (11:null)||2015|
hTERT extends the life of human fibroblasts without compromising type I interferon signaling.
PA3-848 was used in western blot to study the use of human telomerase reverse transcriptase to prolong the life of human fibroblasts whilst retaining their suitability for studying viral replication
|Smith MC,Goddard ET,Perusina Lanfranca M,Davido DJ||PloS one (8:null)||2013|