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Immunofluorescent analysis of p56 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a p56 polyclonal antibody (Product # PA3-848) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA3-848 detects ISG56 (p56) from human and mouse samples.
PA3-848 has been successfully used in Western blot and ICC/IF applications to detect a 39-58 kDa band.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
hTERT extends the life of human fibroblasts without compromising type I interferon signaling.
PA3-848 was used in western blot to study the use of human telomerase reverse transcriptase to prolong the life of human fibroblasts whilst retaining their suitability for studying viral replication
|Smith MC,Goddard ET,Perusina Lanfranca M,Davido DJ||PloS one (8:null)||2013|
GARG-16; Glucocorticoid-attenuated response gene 16 protein; IFI-56K; IFIT-1; IFIT1; interferon, alpha-inducible protein (MW 56kD); Interferon-induced 56 kDa protein; Interferon-induced protein with tetratricopeptide repeats 1; interferon-inducible mRNA 561
C56; G10P1; Garg16; IFI-56; IFI-56K; IFI56; IFIT-1; IFIT1; IFNAI1; ISG56; P56; RNM561