Invitrogen

ITGA2 Monoclonal Antibody (31H4)

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ITGA2 Antibody in Western Blot (WB) — Western blot was performed using Anti- ITGA2 Monoclonal Antibody (31H4) (Product # MA1-26714) and ~ 160 kDa band corresponding to ITGA2 was observed across the cell lines tested. Membrane enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), T-47D (Lane 2) and U-937 (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

MA1-26714

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Flow Cytometry (Flow)	1:25-1:50	-
ELISA (ELISA)	10 µg/mL	-
Immunoprecipitation (IP)	25-50 µg/mL	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	31H4
Immunogen	Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_779707

Product Specific Information

MA1-26714 detects Integrin alpha 2 from human samples.

Target Information

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The fourth blade within the beta-propeller is involved specifically in C3bi recognition by integrin alpha M beta 2.

The Journal of biological chemistry

Li Y,Zhang L

MA1-26714 was used in flow cytometry to determine the function of alpha M beta-propeller in specific ligand recognition by alpha M beta 2.

Fri Sep 05 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: alpha 2 subunit of VLA-2 receptor; BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; human platelet alloantigen system 5; Integrin alpha-2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); platelet antigen Br; platelet glycoprotein GPIa; Platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit; VLA-2 subunit alpha; VLA2 receptor

Gene Aliases: BR; CD49B; GPIa; HPA-5; ITGA2; VLA-2; VLAA2

UniProt ID: (Human) P17301

Entrez Gene ID: (Human) 3673

Molecular Function: integrin