|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Leucocytes from a patient suffering from a LGL type leukaemia.|
|Storage buffer||PBS with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 3 publications below|
CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||8 µg/ml||
Gut homing receptors on CD8 T cells are retinoic acid dependent and not maintained by liver dendritic or stellate cells.
MA1-19559 was used in flow cytometry to elucidate the processes that control enterohepatic T-cell migration
|Eksteen B,Mora JR,Haughton EL,Henderson NC,Lee-Turner L,Villablanca EJ,Curbishley SM,Aspinall AI,von Andrian UH,Adams DH||Gastroenterology (137:320)||2009|
|Not Applicable||Not Cited||
Two-dimensional kinetics regulation of alphaLbeta2-ICAM-1 interaction by conformational changes of the alphaL-inserted domain.
MA1-19559 was used in flow cytometry to study conformational changes of the leukocyte integrin alphaLbeta2
|Zhang F,Marcus WD,Goyal NH,Selvaraj P,Springer TA,Zhu C||The Journal of biological chemistry (280:42207)||2005|
|Not Applicable||Not Cited||
Filarial antigens impair the function of human dendritic cells during differentiation.
MA1-19559 was used in flow cytometry to assess the effect of microfilariae antigen on dendritic cells
|Semnani RT,Sabzevari H,Iyer R,Nutman TB||Infection and immunity (69:5813)||2001|