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Flow cytometry analysis of CD11 alpha in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # MA11A10) at a dilution of 0.5 ug/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rabbit, Hamster, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human 180 kD alpha chain of the CD11alpha complex.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 ug/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA11A10 targets CD11 alpha in FACS applications and shows reactivity with Human and Mouse samples.
The MA11A10 immunogen is human 180 kD alpha chain of the CD11alpha complex.
MA11A10 detects CD11 alpha which has a predicted molecular weight of approximately 126 kDa.
ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inflamed lymphatic endothelium suppresses dendritic cell maturation and function via Mac-1/ICAM-1-dependent mechanism.
MA11A10 was used in blocking or activating experiment to investigate the suppresive effect of inflamed lymphatic endothelium on dendritic cell maturation and its mechanism
|Podgrabinska S,Kamalu O,Mayer L,Shimaoka M,Snoeck H,Randolph GJ,Skobe M||Journal of immunology (Baltimore, Md. : 1950) (183:1767)||2009|
Dual mode of HMG-CoA reductase inhibition on dendritic cell invasion.
MA11A10 was used in blocking/activating experiment to study the mechanism for the effect inhibitor HMG-CoA reductase on dendritic cell invasion
|Kofler S,Schlichting C,Jankl S,Nickel T,Weis M||Atherosclerosis (197:105)||2008|
Adhesion of peripheral blood mononuclear cells and CD4+ T cells from patients with psoriasis to cultured endothelial cells via the interaction between lymphocyte function-associated antigen type 1 and intercellular adhesion molecule 1.
MA11A10 was used in blocking/activating experiment to study the adhesion of PBMC and CD4+ T cells from psoriasis patients to cultured endothelial cells
|Watabe D,Kanno H,Yoshida A,Kurose A,Akasaka T,Sawai T||The British journal of dermatology (157:259)||2007|
Talin1 regulates TCR-mediated LFA-1 function.
MA11A10 was used in blocking/activating experiment to investigate the role of Talin 1 in the regulation of LFA-1 by TCR
|Simonson WT,Franco SJ,Huttenlocher A||Journal of immunology (Baltimore, Md. : 1950) (177:7707)||2006|
Human macrophages rescue myoblasts and myotubes from apoptosis through a set of adhesion molecular systems.
MA11A10 was used in blocking or activating experiment to study the mechanisms by which human macrophages rescue myoblasts and myotubes from apoptosis
|Sonnet C,Lafuste P,Arnold L,Brigitte M,Poron F,Authier FJ,Chrétien F,Gherardi RK,Chazaud B||Journal of cell science (119:2497)||2006|
A novel flow-cytometry-based assay for quantification of affinity and avidity changes of integrins.
MA11A10 was used in blocking/activating experiment to investigate the reliability of a novel flow-cytometry-based assay for quantification of affinity and avidity changes of integrins
|Konstandin MH,Sester U,Klemke M,Weschenfelder T,Wabnitz GH,Samstag Y||Journal of immunological methods (310:67)||2006|
Dendritic cell adhesion is enhanced on endothelial cells preexposed to calcineurin inhibitors.
MA11A10 was used in blocking/activating experiment to investigate the influence of calcineurin inhibitors on dendritic cell adhesion and transmigration to allogeneic endothelial cells
|Schlichting CL,Schareck WD,Weis M||Journal of cardiovascular pharmacology (46:250)||2005|
Transendothelial migration of human basophils.
MA11A10 was used in blocking/activating experiment to investigate the basophil transendothelial migration
|Iikura M,Ebisawa M,Yamaguchi M,Tachimoto H,Ohta K,Yamamoto K,Hirai K||Journal of immunology (Baltimore, Md. : 1950) (173:5189)||2004|
Vav1 phosphorylation is induced by beta2 integrin engagement on natural killer cells upstream of actin cytoskeleton and lipid raft reorganization.
MA11A10 was used in blocking/activating experiment to investigate the role of LFA-1 for the regulation of Vav1 in NK cells
|Riteau B,Barber DF,Long EO||The Journal of experimental medicine (198:469)||2003|
Human natural killer cell activity against porcine targets: modulation by control of the oxidation-reduction environment and role of adhesion molecule interactions.
MA11A10 was used in blocking/activating experiment to investigate the effect of redox in the human organ transplantation from pigs
|Horvath-Arcidiacono JA,Tsuyuki S,Mostowski H,Bloom ET||Cellular immunology (222:35)||2003|
Endothelial determinants of dendritic cell adhesion and migration: new implications for vascular diseases.
MA11A10 was used in blocking/activating experiment to investigate the mechanism for dendritic cell adhesion and migration
|Weis M,Schlichting CL,Engleman EG,Cooke JP||Arteriosclerosis, thrombosis, and vascular biology (22:1817)||2002|
CD2 molecules redistribute to the uropod during T cell scanning: implications for cellular activation and immune surveillance.
MA11A10 was used in blocking/activating experiment to study the redistribution of CD2 to the uropod in T cell scanning
|Tibaldi EV,Salgia R,Reinherz EL||Proceedings of the National Academy of Sciences of the United States of America (99:7582)||2002|
ICAM-3 (CD50) is expressed by human mast cells: induction of homotypic mast cell aggregation via ICAM-3.
MA11A10 was used in blocking/activating experiment to investigate the characteristics of ICAM3 in human mast cells
|Babina M,Mammeri K,Henz BM||Cell adhesion and communication (7:195)||2000|
Respiratory syncytial virus infection enhances neutrophil and eosinophil adhesion to cultured respiratory epithelial cells. Roles of CD18 and intercellular adhesion molecule-1.
MA11A10 was used in blocking/activating experiment to investigate the effect of ICAM 1 and CD18 on the aggregation of white cells with RSV infection
|Stark JM,Godding V,Sedgwick JB,Busse WW||Journal of immunology (Baltimore, Md. : 1950) (156:4774)||1996|
Isolation and characterization of cell lines with genetically distinct mutations downstream of protein kinase C that result in defective activation-dependent regulation of T cell integrin function.
MA11A10 was used in blocking/activating experiment to characterize two cells with defective T cell integrin function
|Mobley JL,Ennis E,Shimizu Y||Journal of immunology (Baltimore, Md. : 1950) (156:948)||1996|
Adhesion of Epstein-Barr virus-positive natural killer cell lines to cultured endothelial cells stimulated with inflammatory cytokines.
MA11A10 was used in flow cytometry to study the effect of inflammatory cytokines on the adhesion of NK cells to endothelial cells
|Kanno H,Watabe D,Shimizu N,Sawai T||Clinical and experimental immunology (151:519)||2008|
Human CD1-restricted T cell recognition of lipids from pollens.
MA11A10 was used in flow cytometry to study the immune response of pollen lipids
|Agea E,Russano A,Bistoni O,Mannucci R,Nicoletti I,Corazzi L,Postle AD,De Libero G,Porcelli SA,Spinozzi F||The Journal of experimental medicine (202:295)||2005|
Monocyte adhesion to xenogeneic endothelium during laminar flow is dependent on alpha-Gal-mediated monocyte activation.
MA11A10 was used in flow cytometry to investigate the mechanism of the monocyte interaction with xenogeneic endothelium
|Peterson MD,Jin R,Hyduk S,Duchesneau P,Cybulsky MI,Waddell TK||Journal of immunology (Baltimore, Md. : 1950) (174:8072)||2005|
CD11b/CD18-coated microspheres attach to E-selectin under flow.
MA11A10 was used in flow cytometry to study the interaction between E-selectin and CD11b/CD18
|Crutchfield KL,Shinde Patil VR,Campbell CJ,Parkos CA,Allport JR,Goetz DJ||Journal of leukocyte biology (67:196)||2000|
Fever-range hyperthermia enhances L-selectin-dependent adhesion of lymphocytes to vascular endothelium.
MA11A10 was used in flow cytometry to investigate the role of L-selectin-dependent adhesion
|Wang WC,Goldman LM,Schleider DM,Appenheimer MM,Subjeck JR,Repasky EA,Evans SS||Journal of immunology (Baltimore, Md. : 1950) (160:961)||1998|
Recruitment of dynein to the Jurkat immunological synapse.
MA11A10 was used in ELISA to investigate the formation of the Jurkat immunological synapse
|Combs J,Kim SJ,Tan S,Ligon LA,Holzbaur EL,Kuhn J,Poenie M||Proceedings of the National Academy of Sciences of the United States of America (103:14883)||2006|
Phenyl methimazole inhibits TNF-alpha-induced VCAM-1 expression in an IFN regulatory factor-1-dependent manner and reduces monocytic cell adhesion to endothelial cells.
MA11A10 was used in ELISA to study the effect of phenyl methimazole on tumor necrosis factor-alpha-induced VCAM-1 expression and its mechanism
|Dagia NM,Harii N,Meli AE,Sun X,Lewis CJ,Kohn LD,Goetz DJ||Journal of immunology (Baltimore, Md. : 1950) (173:2041)||2004|
Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils.
MA11A10 was used in immunoprecipitation to investigate the interaction between BAP31 and CD11b/CD18
|Zen K,Utech M,Liu Y,Soto I,Nusrat A,Parkos CA||The Journal of biological chemistry (279:44924)||2004|
Heterogeneous mutations in the beta subunit common to the LFA-1, Mac-1, and p150,95 glycoproteins cause leukocyte adhesion deficiency.
MA11A10 was used in immunoprecipitation to study the effect of mutations in the LFA-1, Mac-1 and p159 common beta subunit on leukocyte adhesion
|Kishimoto TK,Hollander N,Roberts TM,Anderson DC,Springer TA||Cell (50:193)||1987|
Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family.
MA11A10 was used in immunoprecipitation to purify and clone the beta subunit of LFA-1, Mac-1 and other leukocyte adhesion proteins
|Kishimoto TK,O'Connor K,Lee A,Roberts TM,Springer TA||Cell (48:681)||1987|
A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.
MA11A10 was used in immunoprecipitation to investigate the relationship among the subunits of leukocyte cell surface antigens
|Sanchez-Madrid F,Nagy JA,Robbins E,Simon P,Springer TA||The Journal of experimental medicine (158:1785)||1983|
Mapping the intercellular adhesion molecule-1 and -2 binding site on the inserted domain of leukocyte function-associated antigen-1.
MA11A10 was used in immunocytochemistry to mapp the binding site for ICAM-1 and -2 on LFA-1 inserted domain
|Edwards CP,Fisher KL,Presta LG,Bodary SC||The Journal of biological chemistry (273:28937)||1998|
alpha L integrin; antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; integrin alpha L; integrin alpha-L; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; LFA-1 alpha; LFA-1A; lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1 alpha polypeptide; lymphocyte function-associated antigen 1, alpha polypeptide
(p180); CD11A; ITGAL; LFA-1; LFA-1A; LFA1A; Ly-15; Ly-21