|Tested species reactivity||Bovine, Dog, Cat, Goat, Mustelid, Mink, Pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Affinity purified beta-2 integrins from splenic lysate|
|Storage buffer||tissue culture supernatant diluted in PBS|
|Contains||0.1% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD11b, also known as the alpha M integrin chain or MAC-1, is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
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