|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rheumatoid synovial cells and human monocytes.|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||10 µl for 10^6 cells in 100 µl|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-90974 detects Integrin alpha M from non-human primate, human samples.
MA1-90974 has been successfully used in immunohistochemistry (frozen tissue), immunoprecipitation applications.
The MA1-90974 immunogen is: Rheumatoid synovial cells and human monocytes.
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood.
MA1-90974 was used in flow cytometry to develop a xenotransplantation system using human hematopoietic stem cells and mice
|Park CY,Majeti R,Weissman IL||Nature protocols (3:1932)||2009|
Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins.
MA1-90974 was used in blocking/activating experiment to investigate the effect of beta-glucan for the regulation of neutrophil migration
|Harler MB,Wakshull E,Filardo EJ,Albina JE,Reichner JS||Journal of immunology (Baltimore, Md. : 1950) (162:6792)||1999|