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Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded mouse spleen sections at 400x magnification with DAPI counterstain. Primary antibody used was a CD11b/ITGAM/Integrin alpha M monoclonal antibody (clone M1/70.15) at a 1:500 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-rat at a 1:500 dilution and Normal Goat Serum (blocking)
|Tested species reactivity||Mouse|
|Published species reactivity||Artificial Control , Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||C57BL/6 spleen cell enriched for T lymphocytes|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1 ug/10^6 cells|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17857 targets CD11b/ITGAM/Integrin alpha M in FACS, FN, IHC (F), IHC (P), IP, and WB applications and shows reactivity with Mouse samples.
The MA5-17857 immunogen is c57BL/6 spleen cell enriched for T lymphocytes.
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
M27 expressed by cytomegalovirus counteracts effective type I interferon induction of myeloid cells but not of plasmacytoid dendritic cells.
MA5-17857 was used in flow cytometry to test if professional antigen-presenting cell subsets and MCMV-encoded evasins alter IFN-I responses
|Döring M,Lessin I,Frenz T,Spanier J,Kessler A,Tegtmeyer P,Dağ F,Thiel N,Trilling M,Lienenklaus S,Weiss S,Scheu S,Messerle M,Cicin-Sain L,Hengel H,Kalinke U||Journal of virology (88:13638)||2014|
Enhanced resistance against Listeria monocytogenes achieved by pretreatment with granulocyte colony-stimulating factor.
MA5-17857 was used in flow cytometry to study the roles of G-CSF and polymorphonuclear leukocytes in Listeria monocytogenes-infected mice.
|Shinomiya N,Tsuru S,Katsura Y,Kayashima S,Nomoto K||Infection and immunity (59:4740)||1991|
|Artificial Control||Not Cited||
TNF regulates chemokine induction essential for cell recruitment, granuloma formation, and clearance of mycobacterial infection.
MA5-17857 was used in flow cytometry to assess the role of TNF in the formation and maintenance of granulomas.
|Roach DR,Bean AG,Demangel C,France MP,Briscoe H,Britton WJ||Journal of immunology (Baltimore, Md. : 1950) (168:4620)||2002|
CD11 antigen-like family member B; CD11B; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; CR3A; Integrin alpha M; integrin alpha-M; leukocyte adhesion receptor MO1; MAC-1; Mac-1 alpha; MAC1A; macrophage antigen alpha; MGC117044; MO1A; SLEB6
Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1