|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Immunogen||C57BL/10 spleen cell enriched for T lymphocytes|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.2 ug/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17858 targets CD11b/ITGAM/Integrin alpha M in FACS, IHC (F), and IHC (P) applications and shows reactivity with Mouse samples.
The MA5-17858 immunogen is c57BL/10 spleen cell enriched for T lymphocytes.
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The presence of IL-17A and T helper 17 cells in experimental mouse brain tumors and human glioma.
MA5-17858 was used in immunohistochemistry - frozen section to study Th17 cells in glia-derived tumors of the central nervous system
|Wainwright DA,Sengupta S,Han Y,Ulasov IV,Lesniak MS||PloS one (5:null)||2010|
Host conditioning with total lymphoid irradiation and antithymocyte globulin prevents graft-versus-host disease: the role of CD1-reactive natural killer T cells.
MA5-17858 was used in flow cytometry to test if a total lymphoid irradiation and anti-thymocyte serum regimen can prevent graft verses host disease.
|Lan F,Zeng D,Higuchi M,Higgins JP,Strober S||Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation (9:355)||2003|
Predominance of NK1.1+TCR alpha beta+ or DX5+TCR alpha beta+ T cells in mice conditioned with fractionated lymphoid irradiation protects against graft-versus-host disease: "natural suppressor" cells.
MA5-17858 was used in flow cytometry to develop a nonmyeloablative host conditioning regimen for transplantation.
|Lan F,Zeng D,Higuchi M,Huie P,Higgins JP,Strober S||Journal of immunology (Baltimore, Md. : 1950) (167:2087)||2001|
Transgenic expression of CD95 ligand on thyroid follicular cells confers immune privilege upon thyroid allografts.
MA5-17858 was used in flow cytometry to report that ectopic FasL expression prevents thyroid allograft rejection.
|Tourneur L,Malassagne B,Batteux F,Fabre M,Mistou S,Lallemand E,Lores P,Chiocchia G||Journal of immunology (Baltimore, Md. : 1950) (167:1338)||2001|
Peritoneal B-cell development depends on strain, radiation, and time.
MA5-17858 was used in flow cytometry to study generation of peritoneal B-1a cells after bone marrow transfer.
|Stickler M,Harding F||Experimental hematology (29:221)||2001|
Surface antigen expression in spleen cells of C57B1/6 mice during ageing: influence of sex and parity.
MA5-17858 was used in flow cytometry to test if pregnancies and gender influence the cellar changes observed during ageing in mice.
|Barrat F,Lesourd BM,Louise A,Boulouis HJ,Vincent-Naulleau S,Thibault D,Sanaa M,Neway T,Pilet CH||Clinical and experimental immunology (107:593)||1997|
|Not Applicable||Not Cited||
Chemokine receptor CXCR5 supports solitary intestinal lymphoid tissue formation, B cell homing, and induction of intestinal IgA responses.
MA5-17858 was used in flow cytometry to characterize and track solitary intestinal lymphoid tissues
|Velaga S,Herbrand H,Friedrichsen M,Jiong T,Dorsch M,Hoffmann MW,Förster R,Pabst O||Journal of immunology (Baltimore, Md. : 1950) (182:2610)||2009|
|Not Applicable||1 µg/ml||
Lack of antigen-specific tissue remodeling in mice deficient in the macrophage galactose-type calcium-type lectin 1/CD301a.
MA5-17858 was used in flow cytometry to investigate the contribution of macrophage galactose-type C-type lectin1 to granulation tissue formation
|Sato K,Imai Y,Higashi N,Kumamoto Y,Onami TM,Hedrick SM,Irimura T||Blood (106:207)||2005|
|Not Applicable||Not Cited||
Granulation tissue formation by nonspecific inflammatory agent occurs independently of macrophage galactose-type C-type lectin-1.
MA5-17858 was used in immunohistochemistry - frozen section to determine the role of a macrophage galactose-type calcium-type lectin-1 in antigen-independent granulation tissue formation
|Sato K,Komatsu N,Higashi N,Imai Y,Irimura T||Clinical immunology (Orlando, Fla.) (115:47)||2005|